Enhanced sensitivity of lateral flow tests using a two-dimensional paper network format.
Enhanced sensitivity of lateral flow tests using a two-dimensional paper network format.
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DOI：
10.1021/ac201950g
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发表时间：
2011-10-15
影响因子：
7.4
通讯作者：
Yager, Paul
中科院分区：
文献类型：
--
作者：
Fu, Elain;Liang, Tinny;Houghtaling, Jared;Ramachandran, Sujatha;Ramsey, Stephen A.;Lutz, Barry;Yager, Paul
在低资源环境中需要快速、易用和低成本的护理点诊断分析；侧向流动试验已成为此类环境中的标准生物分析形式，因为它符合这些标准。然而，对于许多分析物，传统的侧向流动试验缺乏具有临床实用价值所需的灵敏度。为了解决这一限制，我们正在开发一个纸质网络平台，将传统的横向流动测试扩展到二维。这种二维结构允许结合多步骤过程以提高灵敏度，同时仍然保留了传统侧向流动测试的积极方面。在这里，我们创建了一种易于使用的、信号放大的免疫分析方法，它基于一种改进的人绒毛膜促性腺激素商业试纸试验，这种激素用于检测怀孕，并证明了与传统的侧向流动分析方法相比，检测极限有所提高。这些结果突出了纸质网络平台在发达国家和发展中国家在低资源环境下加强获得高质量诊断能力的潜力。
Point-of-care diagnostic assays that are rapid, easy-to-use, and low-cost are needed for use in low-resource settings; the lateral flow test has become the standard bioassay format in such settings because it meets those criteria. However, for a number of analytes, conventional lateral flow tests lack the sensitivity needed to have clinical utility. To address this limitation, we are developing a paper network platform that extends the conventional lateral flow test to two dimensions. The two-dimensional structures allow incorporation of multi-step processes for improved sensitivity, while still retaining the positive aspects of conventional lateral flow tests. Here we create an easy-to-use, signal-amplified immunoassay based on a modified commercial strip test for human chorionic gonadotropin, the hormone used to detect pregnancy, and demonstrate an improved limit of detection compared to a conventional lateral flow assay. These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds.
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