Effect of capsid confinement on the chromatin organization of the SV40 minichromosome.
Effect of capsid confinement on the chromatin organization of the SV40 minichromosome.
复制标题
DOI：
10.1093/nar/gks1270
复制
发表时间：
2013-02-01
影响因子：
14.9
通讯作者：
Harries D
中科院分区：
文献类型：
--
作者：
Saper G;Kler S;Asor R;Oppenheim A;Raviv U;Harries D
利用小角X射线散射，我们确定了SV40病毒内微染色体的三维堆积结构。在溶液中，微染色体由封闭的环状dsDNA组成，在核小体中络合，结构类似于细胞染色质。相反，我们发现了一种独特的纳米包裹染色质的组织，即微染色体密度在衣壳的中心略高，并向壁上降低。这个组织与一个粗粒度的计算机模型非常一致，该模型解释了病毒衣壳限制下的系留核小体相互作用。与受限液晶类似，但与细胞染色质的螺线管结构相反，我们的模拟表明衣壳内的核小体缺乏取向有序。然而，与衣壳壁相邻的层中的核小体与边界对齐，从而导致染色质的“熔滴”状态。这些发现表明，核小体相互作用足以预测多瘤病毒衣壳中的基因组组织，并强调真核细胞染色质结构对纳米尺度限制的适应性。
Using small-angle X-ray scattering, we determined the three-dimensional packing architecture of the minichromosome confined within the SV40 virus. In solution, the minichromosome, composed of closed circular dsDNA complexed in nucleosomes, was shown to be structurally similar to cellular chromatin. In contrast, we find a unique organization of the nanometrically encapsidated chromatin, whereby minichromosomal density is somewhat higher at the center of the capsid and decreases towards the walls. This organization is in excellent agreement with a coarse-grained computer model, accounting for tethered nucleosomal interactions under viral capsid confinement. With analogy to confined liquid crystals, but contrary to the solenoid structure of cellular chromatin, our simulations indicate that the nucleosomes within the capsid lack orientational order. Nucleosomes in the layer adjacent to the capsid wall, however, align with the boundary, thereby inducing a ‘molten droplet’ state of the chromatin. These findings indicate that nucleosomal interactions suffice to predict the genome organization in polyomavirus capsids and underscore the adaptable nature of the eukaryotic chromatin architecture to nanoscale confinement.
登录
查看更多内容
影响因子：
3.4
作者：
Depken, Martin;Schiessel, Helmut
通讯作者：
Schiessel, Helmut
影响因子：
8.6
作者：
Galanis J;Nossal R;Losert W;Harries D
通讯作者：
Harries D
影响因子：
1.7
作者：
EMERSON, APJ;LUCKHURST, GR;WHATLING, SG
通讯作者：
WHATLING, SG
影响因子：
2.9
作者：
Bertin, A;Leforestier, A;Livolant, F
通讯作者：
Livolant, F
影响因子：
3.4
作者：
Bertin, Aurelie;Mangenot, Stephanie;Livolant, Francoise
通讯作者：
Livolant, Francoise