Temperature Differentially Influences the Capacity of Trichoderma Species to Induce Plant Defense Responses in Tomato Against Insect Pests.
Temperature Differentially Influences the Capacity of Trichoderma Species to Induce Plant Defense Responses in Tomato Against Insect Pests.
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DOI：
10.3389/fpls.2021.678830
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发表时间：
2021
影响因子：
5.6
通讯作者：
Digilio MC
中科院分区：
文献类型：
--
作者：
Di Lelio I;Coppola M;Comite E;Molisso D;Lorito M;Woo SL;Pennacchio F;Rao R;Digilio MC
木霉是一种生态机会性、无毒的真菌，因其具有保护作物免受病原菌侵袭和促进植物生长的能力，在农业上得到了广泛的应用。最近，有研究表明，它们也可能具有互补的特性，增强植物对昆虫的防御屏障。然而，这些真菌的使用在某种程度上受到其生物控制活性的不同程度的破坏，而生物控制活性受环境条件的影响。了解这种变异性的来源对于它在植物保护中的有利可图和广泛使用至关重要。在这里，我们重点研究了温度对非洲哈茨木霉T22、萎缩木霉菌P1的影响以及昆虫诱导番茄的防御反应。这两个菌株的体外发育受温度的影响不同，观察到的模式与它们在番茄植株上诱导的对美洲斑潜蝇和夜蛾的抗性水平随温度的变化一致。在25℃下，黑曲霉T22处理的番茄植株对这两种害虫表现出较强的抗性，而阿托维里木霉P1在20℃时表现得更有效。通过比较这两种木霉产生的植物转录图谱，可以确定参与观察到的反应的特定防御基因，并利用实时定量逆转录聚合酶链式反应(qRT-PCR)来评估木霉菌处理的番茄植株在两种显著影响真菌生物学行为的温度条件下的差异基因表达。这些结果将有助于为合理选择最适合田间应用的木霉菌株铺平道路，以便最好地应对当地环境条件和全球变暖造成的极端气候变化带来的挑战。
Species of the ecological opportunistic, avirulent fungus, Trichoderma are widely used in agriculture for their ability to protect crops from the attack of pathogenic fungi and for plant growth promotion activity. Recently, it has been shown that they may also have complementary properties that enhance plant defense barriers against insects. However, the use of these fungi is somewhat undermined by their variable level of biocontrol activity, which is influenced by environmental conditions. Understanding the source of this variability is essential for its profitable and wide use in plant protection. Here, we focus on the impact of temperature on Trichoderma afroharzianum T22, Trichoderma atroviride P1, and the defense response induced in tomato by insects. The in vitro development of these two strains was differentially influenced by temperature, and the observed pattern was consistent with temperature-dependent levels of resistance induced by them in tomato plants against the aphid, Macrosiphum euphorbiae, and the noctuid moth, Spodoptera littoralis. Tomato plants treated with T. afroharzianum T22 exhibited enhanced resistance toward both insect pests at 25°C, while T. atroviride P1 proved to be more effective at 20°C. The comparison of plant transcriptomic profiles generated by the two Trichoderma species allowed the identification of specific defense genes involved in the observed response, and a selected group was used to assess, by real-time quantitative reverse transcription PCR (qRT-PCR), the differential gene expression in Trichoderma-treated tomato plants subjected to the two temperature regimens that significantly affected fungal biological performance. These results will help pave the way toward a rational selection of the most suitable Trichoderma isolates for field applications, in order to best face the challenges imposed by local environmental conditions and by extreme climatic shifts due to global warming.
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影响因子：
7.4
作者：
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通讯作者：
Goggin, Fiona L.
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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