Differences in cerebral blood vasculature and flow in awake and anesthetized mouse cortex revealed by quantitative optical coherence tomography angiography.
Differences in cerebral blood vasculature and flow in awake and anesthetized mouse cortex revealed by quantitative optical coherence tomography angiography.
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DOI：
10.1016/j.jneumeth.2021.109094
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发表时间：
2021-04-01
影响因子：
3
通讯作者：
Wang RK
中科院分区：
文献类型：
--
作者：
Rakymzhan A;Li Y;Tang P;Wang RK
大多数体内神经血管成像研究是在麻醉动物中进行的。然而，麻醉显著影响脑血流动力学。应用光学相干断层扫描（OCT）方法，如光学微血管成像（OMAG）和多普勒光学微血管成像（DOMAG），定量评价麻醉对小鼠大脑血管和血流的影响。OMAG结果显示氯胺酮-噻嗪和异氟醚诱导大鼠大血管直径和毛细血管密度增加，说明两种麻醉药均有血管舒张作用。此外，DOMAG的初步结果显示，异氟醚增加了基线脑血流量。与其他体内成像方式相比，OCT可以提供无标记的皮质组织评估，包括组织形态、脑血管网络和血流信息，低至毛细血管水平，视野大，成像速度快。OCT血管造影方法证明了能够测量清醒和麻醉小鼠之间大脑血管和毛细血管网络的基线形态学和血流参数的差异。
Most of the in vivo neurovascular imaging studies are performed in anesthetized animals. However, anesthesia significantly affects cerebral hemodynamics. We applied optical coherence tomography (OCT) methods such as optical microangiography (OMAG) and Doppler optical microangiography (DOMAG) to quantitatively evaluate the effect of anesthesia in cerebral vasculature and blood flow in mouse brain. The OMAG results indicated the increase of large vessel diameter and capillary density induced by ketamine-xylazine and isoflurane, meaning that both anesthetics caused vasodilation. In addition, the preliminary results from DOMAG showed that isoflurane increased the baseline cerebral blood flow. In comparison with other in vivo imaging modalities, OCT can provide label-free assessment of cortical tissue including tissue morphology, cerebral blood vessel network and flow information down to capillary level, with a large field of view and high imaging speed. OCT angiography methods demonstrated the ability to measure the differences in the baseline morphological and flow parameters of both large and capillary cerebrovascular networks between awake and anesthetized mice.
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影响因子：
3
作者：
Li Y;Choi WJ;Qin W;Baran U;Habenicht LM;Wang RK
通讯作者：
Wang RK
影响因子：
4.6
作者：
Sharp PS;Shaw K;Boorman L;Harris S;Kennerley AJ;Azzouz M;Berwick J
通讯作者：
Berwick J
影响因子：
2.2
作者：
Szabo, C. Akos;Narayana, Shalini;Franklin, Crystal;Knape, Koyle D.;Davis, M. Duff;Fox, Peter T.;Leland, M. Michelle;Williams, Jeff T.
通讯作者：
Williams, Jeff T.
影响因子：
3
作者：
Santisakultarm TP;Kersbergen CJ;Bandy DK;Ide DC;Choi SH;Silva AC
通讯作者：
Silva AC
DOI：
10.1097/01.wcb.0000054755.93668.20
发表时间：
2003-04-01
影响因子：
6.3
作者：
Sicard, K;Shen, Q;Duong, TQ
通讯作者：
Duong, TQ