Optical coherence tomography based microangiography provides an ability to longitudinally image arteriogenesis in vivo.
Optical coherence tomography based microangiography provides an ability to longitudinally image arteriogenesis in vivo.
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DOI：
10.1016/j.jneumeth.2016.10.010
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发表时间：
2016-12-01
影响因子：
3
通讯作者：
Wang RK
中科院分区：
文献类型：
--
作者：
Li Y;Choi WJ;Qin W;Baran U;Habenicht LM;Wang RK
动脉发生是指预先存在的侧支小动脉的活跃生长，这是闭塞性血管疾病中至关重要的组织保存过程。我们建议使用基于光学相干断层扫描（OCT）的微血管造影（OMAG）来监测小鼠耳动脉横断和小鼠脑局灶性中风后的动脉发生。我们对小鼠耳的纵向研究表明，动脉生成的生长期发生在血管闭塞后12-24小时，以侧支血管直径和速度的变化为指标。此外，通过组织厚度测量和oct的淋巴管信号判断，局部炎症的程度与动脉形成的时间过程一致。在小鼠脑研究中，识别了侧支血管形态、血流速度和方向，并观察到卒中时小动脉-小动脉吻合处（AAA）侧支血流的激活。与组织学和荧光成像相比，OCT/OMAG是完全无创的，能够产生一致的侧枝血管形态和血管动力学的纵向变化结果。OCT/OMAG是一种很有前途的小动物侧支血管重构纵向研究成像工具。该技术可用于指导动脉生成刺激治疗闭塞性血管疾病（包括中风）的体内实验。
Arteriogenesis describes the active growth of the pre-existing collateral arterioles, which is a crucial tissue-saving process in occlusive vascular diseases. We propose to use optical coherence tomography (OCT)-based microangiography (OMAG) to monitor arteriogenesis following artery transection in mouse ear and focal stroke in mouse brain. Our longitudinal mouse ear study shows that the growth phase of arteriogenesis, indicated by changes in collateral vessel diameter and velocity, occurs between 12-24 hours after vessel occlusion. Additionally, the magnitude of local inflammation is consistent with the time course of arteriogenesis, judging by the tissue thickness measurement and lymphatic vessel signals in OCT. In the mouse brain study, collateral vessel morphology, blood flow velocity and directionality are identified, and an activation of the collateral flow at the arteriolo-arteriolar anastomoses (AAA) is observed during stroke. In comparison with histology and fluorescence imaging, OCT/OMAG is completely non-invasive and capable of producing consistent results of longitudinal changes in collateral vessel morphology and vasodynamics. OCT/OMAG is a promising imaging tool for longitudinal study of collateral vessel remodeling in small animals. This technique can be applied in guiding the in vivo experiments of arteriogenesis stimulation to treat occlusive vascular diseases, including stroke.
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