Green fluorescent proteins are light-induced electron donors.

Green fluorescent proteins are light-induced electron donors.
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DOI:
10.1038/nchembio.174
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发表时间:
2009-07
影响因子:
14.8
通讯作者:
Lukyanov, Konstantin A.
Lukyanov, Konstantin A.
中科院分区:
生物学1区
文献类型:
--
作者:
Bogdanov, Alexey M.;Mishin, Alexander S.;Yampolsky, Ilia V.;Belousov, Vsevolod V.;Chudakov, Dmitriy M.;Subach, Fedor V.;Verkhusha, Vladislav V.;Lukyanov, Sergey;Lukyanov, Konstantin A.

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绿色荧光蛋白(GFP)家族的蛋白质由于其独特的生物化学和作为体内标记物的广泛用途而众所周知。在这里,我们发现了一个新的功能,不同来源的GFPs作为光诱导的电子供体与各种电子受体,包括生物相关的光化学反应。此外,这个过程伴随着绿色到红色的GFP光转换可以在活细胞中观察到,而无需额外的处理。
Proteins of the green fluorescent protein (GFP) family are well known due to their unique biochemistry and extensive use as in vivo markers. Here, we discovered a new feature of GFPs of diverse origins to act as the light-induced electron donors in photochemical reactions with various electron acceptors, including biologically relevant ones. Moreover, this process accompanying with green-to-red GFP photoconversion can be observed in living cells without additional treatment.
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