Extra c-myc oncogene copies in high risk cutaneous malignant melanoma and melanoma metastases.
Extra c-myc oncogene copies in high risk cutaneous malignant melanoma and melanoma metastases.
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DOI：
10.1054/bjoc.2000.1535
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发表时间：
2001-01-05
影响因子：
8.8
通讯作者：
Peter RU
中科院分区：
文献类型：
--
作者：
Kraehn GM;Utikal J;Udart M;Greulich KM;Bezold G;Kaskel P;Leiter U;Peter RU
Amplification and overexpression of the c-myc gene have been associated with neoplastic transformation in a plethora of malignant tumours. We applied interphase fluorescence in situ hybridization (FISH) with a locus-specific probe for the c-myc gene (8q24) in combination with a corresponding chromosome 8 α-satellite probe to evaluate genetic alterations in 8 primary melanomas and 33 advanced melanomas and compared it to 12 melanocytic nevi, 7 safety margins and 2 cases of normal skin. Additionally, in metaphase spreads of 7 melanoma cell lines a whole chromosome 8 paint probe was used. We investigated the functionality of the c-myc gene by detecting c-myc RNA expression with RT-PCR and c-myc protein by immunohistochemistry. 4/8 primary melanomas and 11/33 melanoma metastases showed additional c-myc signals relative to the centromere of chromosome 8 copy number. None of the nevi, safety margins or normal skin samples demonstrated this gain. In 2/7 melanoma cell lines (C32 and WM 266–4) isochromosome 8q formation with a relative gain of c-myc copies and a loss of 8p was observed. The highest c-myc gene expression compared to GAPDH was found in melanoma metastases (17.5%). Nevi (6.6%) and primary melanomas (5.0%) expressed the c-myc gene on a lower level. 72.7% of the patients with c-myc extra copies had visceral melanoma metastases (UICC IV), patients without c-myc gain in 35.0% only. The collective with additional c-myc copies also expressed the gene on a significantly higher level. These results indicate that a c-myc gain in relation to the centromere 8 copy number might be associated with advanced cutaneous melanoma. © 2001 Cancer Research Campaign http://www.bjcancer.com
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影响因子：
8.8
作者：
Masramon, L;Arribas, R;Tortola, S;Perucho, M;Peinado, M A
通讯作者：
Peinado, M A
DOI：
10.1073/pnas.83.9.2934
发表时间：
1986-05-01
影响因子：
11.1
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VLOEMANS, M
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作者：
Schlagbauer-Wadl, H;Griffioen, M;Jansen, B
通讯作者：
Jansen, B