Electrospun Cellulose-Silk Composite Nanofibres Direct Mesenchymal Stem Cell Chondrogenesis in the Absence of Biological Stimulation
Electrospun Cellulose-Silk Composite Nanofibres Direct Mesenchymal Stem Cell Chondrogenesis in the Absence of Biological Stimulation
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电纺纤维素-丝复合纳米纤维在没有生物刺激的情况下直接间充质干细胞软骨形成
DOI：
10.1101/434316
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Begum R
中科院分区：
文献类型：
--
作者：
Begum R
具有固有刺激能力的智能生物材料可以代替生物提示引发特定行为，这将被证明对再生医学应用有利。将天然高分子纤维素和蚕丝混合制成膜，在体外培养条件下可促进人骨髓间充质干细胞（hMSCs）向软骨细胞分化。然而，这种生物材料用于软骨组织工程的真正潜力可以在其三维制造后实现。在这项工作中，我们采用静电纺丝技术来模拟体内的纳米纤维细胞外基质（ECM）。纤维素和丝聚合物在质量比为75：25的再生使用三氟乙酸和乙酸共溶剂系统。这种天然聚合物复合材料首次被直接静电纺丝成纳米纤维，而无需后纺处理。纤维珠的存在和尺寸受环境湿度的影响。再生的复合材料保留了其各自组分的关键化学功能。证明了天然聚合物复合材料与hMSCs的生物相容性，并证实了其在不存在刺激生长因子的情况下指导软骨形成干细胞分化的固有能力。使用成纤维细胞生长因子-2（FGF-2）（一种用于增强hMSC增殖的生长因子），生物化学地对抗这种物理软骨形成刺激。新制造的支架为设计用于软骨组织工程的稳健、自诱导和成本效益高的仿生生物材料提供了基础。
Smart biomaterials with an inherent stimulating capacity that elicit specific behavioursin lieuof biological prompts would prove advantageous for regenerative medicine applications. Specific blends of the natural polymers cellulose and silk cast as films can drive the chondrogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) uponin vitroculture. However, the true potential of such biomaterials for cartilage tissue engineering can be realised upon its three-dimensional fabrication. In this work we employ an electrospinning technique to model thein vivonanofibrous extracellular matrix (ECM). Cellulose and silk polymers at a mass ratio of 75:25 were regenerated using a trifluoroacetic acid and acetic acid cosolvent system. This natural polymer composite was directly electrospun for the first time, into nanofibers without post-spun treatment. The presence and size of fibre beading was influenced by environmental humidity. The regenerated composite retained the key chemical functionalities of its respective components. Biocompatibility of the natural polymer composite with hMSCs was demonstrated and its inherent capacity to direct chondrogenic stem cell differentiation, in the absence of stimulating growth factors, was confirmed. This physical chondrogenic stimulation was countered biochemically using fibroblast growth factor-2 (FGF-2), a growth factor used to enhance the proliferation of hMSCs. The newly fabricated scaffold provides the foundation for designing a robust, self-inductive, and cost-effective biomimetic biomaterial for cartilage tissue engineering.
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影响因子：
4.6
作者：
S. Zarkoob;R. Eby;D. Reneker;S. D. Hudson;Dale Ertley;W. W. Adams-W.
通讯作者：
S. Zarkoob;R. Eby;D. Reneker;S. D. Hudson;Dale Ertley;W. W. Adams-W.
影响因子：
2.3
作者：
Ohkawa, Kousaku;Hayashi, Shinya;Ducreux, Jeremy
通讯作者：
Ducreux, Jeremy
DOI：
10.1302/0301-620x.52b3.554
发表时间：
1970-08
期刊：
The Journal of bone and joint surgery. British volume
影响因子：
--
作者：
H. Muir;P. Bullough;A. Maroudas
通讯作者：
H. Muir;P. Bullough;A. Maroudas
DOI：
10.1002/jbm.10151
发表时间：
2002-08-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Kowalczynska, HM;Nowak-Wyrzykowska, M;Marciniak, E
通讯作者：
Marciniak, E
影响因子：
3
作者：
Nezarati, Roya M.;Eifert, Michelle B.;Cosgriff-Hernandez, Elizabeth
通讯作者：
Cosgriff-Hernandez, Elizabeth