GbSOBIR1 confers Verticillium wilt resistance by phosphorylating the transcriptional factor GbbHLH171 in Gossypium barbadense.
GbSOBIR1 confers Verticillium wilt resistance by phosphorylating the transcriptional factor GbbHLH171 in Gossypium barbadense.
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DOI：
10.1111/pbi.12954
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发表时间：
2019-01
影响因子：
13.8
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Zhou Y;Sun L;Wassan GM;He X;Shaban M;Zhang L;Zhu L;Zhang X
受体样激酶（RLK）是植物先天免疫的重要组成部分。尽管最近的研究表明，BIR 1 - 1（SOBIR 1）的RLK抑制因子可以与多种受体样蛋白相互作用，并且是抵抗真菌病原体所必需的，但信号如何转导并触发免疫反应仍然是个谜。在本研究中，我们从海岛棉中鉴定了一个与防御相关的RLK（命名为GbSOBIR 1），并研究了其功能机制。GbSOBIR 1基因在棉花植物中普遍存在，并通过大丽轮枝菌接种诱导表达。通过病毒诱导的基因沉默敲低GbSOBIR 1导致棉花植物对大丽轮枝菌的抗性减弱，而拟南芥中GbSOBIR 1的异源过表达提高了抗性。我们还发现GbSOBIR 1的激酶区域与酵母双杂交筛选中鉴定为GbbHLH 171的碱性螺旋-环-螺旋（bHLH）转录因子相互作用。GbbHLH 171与GbSOBIR 1在体内和体外均能相互作用并被GbSOBIR 1磷酸化，对棉花抗黄萎病有积极作用。此外，我们发现，这种磷酸化是必不可少的转录活性和功能作用的GbbHLH 171。我们还通过光谱分析和定点突变表明，Ser 413是GbbHLH 171的GbSOBIR 1介导的磷酸化位点。这些结果表明，GbSOBIR 1与GbbHLH 171相互作用，并在棉花对大丽轮枝菌的抗性中起关键作用。
Receptor‐like kinases (RLKs) are important components of plant innate immunity. Although recent studies have revealed that the RLK suppressor of BIR1‐1 (SOBIR1) can interact with multiple receptor‐like proteins and is required for resistance against fungal pathogens, how the signal is transduced and triggers immune responses remains enigmatic. In this study, we identified a defence‐related RLK from Gossypium barbadense (designated GbSOBIR1) and investigated its functional mechanism. Expression of the GbSOBIR1 gene is ubiquitous in cotton plants and is induced by Verticillium dahliae inoculation. Knock‐down of GbSOBIR1 by virus‐induced gene silencing resulted in attenuated resistance of cotton plants to V. dahliae, while heterologous overexpression of GbSOBIR1 in Arabidopsis improves resistance. We also found that the kinase region of GbSOBIR1 interacts with a basic helix‐loop‐helix (bHLH) transcription factor identified as GbbHLH171 in a yeast‐two‐hybrid screen. GbbHLH171 could interact with and be phosphorylated by GbSOBIR1 in vitro and in vivo and contributes positively to the resistance of cotton against V. dahliae. Furthermore, we found that this phosphorylation is essential to the transcriptional activity and functional role of GbbHLH171. We also show by spectrometric analysis and site‐directed mutagenesis that Ser413 is the GbSOBIR1‐mediated phosphorylation site of GbbHLH171. These results demonstrate that GbSOBIR1 interacts with GbbHLH171 and plays a critical role in cotton resistance to V. dahliae.
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