The essential role of Mkx in periodontal ligament on the metabolism of alveolar bone and cementum.
The essential role of Mkx in periodontal ligament on the metabolism of alveolar bone and cementum.
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DOI：
10.1016/j.reth.2023.12.007
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发表时间：
2024-03
影响因子：
4.3
通讯作者：
Asahara, Hiroshi
中科院分区：
文献类型：
--
作者：
Yagasaki, Lisa;Chiba, Tomoki;Kurimoto, Ryota;Nakajima, Mitsuyo;Iwata, Takanori;Asahara, Hiroshi
牙周组织是一种结缔组织，由牙周韧带、牙槽骨、牙骨质和牙龈组成。牙周韧带（PDL）是一种特殊的结缔组织，连接覆盖在牙齿表面的牙骨质和牙槽骨。莫霍克同源盒（Mohawk homeobox, Mkx）是一种在PDL中表达的转录因子，在PDL的发育和稳态中起着至关重要的作用。牙周韧带中Mkx对牙槽骨和牙骨质代谢的详细功能分析尚未进行。采用微型计算机断层扫描（Micro-CT）和3DBon对7周龄雄性野生型（Mkx+/+） （n = 10）和Mkx敲除（Mkx−/−）（n = 6）大鼠的牙槽骨高度、骨密度（BMD）和骨体积分数（bone volume/Total volume: BV/TV）进行测量和分析。对5、6、7周龄Mkx+/+和Mkx−/−大鼠进行苏木精和伊红（H&E）、抗酒石酸酸性磷酸酶（TRAP）、碱性磷酸酶（ALP）和马松三色染色。对5、6和7周龄Mkx+/+和Mkx - / -大鼠（各n = 3）的骨质表面积和trap阳性破骨细胞数量进行量化、测量和比较。Mkx−/−大鼠的牙槽骨高度水平明显高于Mkx+/+大鼠。另一方面，Mkx - / -牙槽骨BMD明显减少。与同年龄的Mkx+/+大鼠相比，Mkx−/−大鼠早在5周时就可以观察到细胞骨质的显著增加。Mkx−/−大鼠中观察到更多trap阳性破骨细胞。我们的研究结果进一步揭示了Mkx在牙周组织稳态中的重要作用。Mkx被发现有助于骨和骨质代谢，可能对牙周炎等疾病的预防至关重要，并可能在再生治疗中显示出潜力。
The periodontium is a connective tissue which consists of periodontal ligament, alveolar bone, cementum and gingiva. Periodontal ligament (PDL) is a specialized connective tissue that connects the cementum – coating the surface of the tooth – to the alveolar bone. Mohawk homeobox (Mkx) is a transcription factor that is expressed in PDL, that is known to play a vital role in the development and homeostasis of PDL. A detailed functional analysis of Mkx in the periodontal ligament for alveolar bone and cementum metabolism has not yet been conducted. Alveolar bone height, bone mineral density (BMD) and bone volume fractions (Bone volume/Total volume: BV/TV) were measured and analyzed using micro-computed tomography (Micro-CT) and 3DBon on 7-week-old male wild-type (WT) (Mkx+/+) (n = 10) and Mkx-knockout (Mkx−/−) (n = 6) rats. Hematoxylin and Eosin (H&E), tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP) and Masson Trichrome staining were performed on 5, 6, and 7-week-old Mkx+/+ and Mkx−/− rats. Cementum surface area and the number of TRAP-positive osteoclasts/mm were quantified, measured, and compared for 5,6 and 7-week-old Mkx+/+ and Mkx−/− rats (n = 3 each). The level of alveolar bone height was significantly higher in Mkx−/− rats than in Mkx+/+ rats. On the other hand, there was significantly less BMD in Mkx−/− alveolar bone. A significant increase in cellular cementum could be observed as early as 5 weeks in Mkx−/− rats when compared with Mkx+/+ rats of the same age. More TRAP-positive osteoclasts were observed in Mkx−/− rats. Our findings further reveal the essential roles of Mkx in the homeostasis of the periodontal tissue. Mkx was found to contribute to bone and cementum metabolism and may be essential to the prevention of diseases such as periodontitis, and could show potential in regenerative treatments.
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