Molecular localization and state of amphotericin B in PEG liposomes.
Molecular localization and state of amphotericin B in PEG liposomes.
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PEG 脂质体中两性霉素 B 的分子定位和状态。
DOI：
10.1016/s0378-5173(99)00323-3
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发表时间：
1999
影响因子：
5.8
通讯作者：
M. Iwatsuru
中科院分区：
文献类型：
--
作者：
K. Moribe;K. Maruyama;M. Iwatsuru
研究了聚乙二醇脂质体中两性霉素B(AmB)的分子定位和状态。用9%蔗糖溶液水合挤压法制备了由二棕榈酰磷脂酰胆碱(DPPC)、胆固醇(CH)和二硬脂酰基-N-(单甲氧基聚乙二醇琥珀酰基)磷脂酰乙醇胺(DSPE-PEG2000)组成的聚乙二醇单硬脂酰胆碱(DPPC)-聚乙二醇乙醇胺(DSPE-PEG，平均相对分子质量为2000)的聚乙二醇单硬脂酰胆碱(DPPC)、胆固醇(CH)和双硬脂酰基-N-(单甲氧基聚乙二醇丁二醇基)磷脂酰乙醇胺(DSPE-PEG，平均相对分子质量为2000)的聚乙二醇单硬脂酰胆碱(DPPC)脂质体。载药量随DSPE-PEG的掺入而增加，随CH的掺入而减少。通过聚乙二醇/葡聚糖两相分配、钾渗透率、荧光猝灭和圆二色谱(CD)研究了AMB的分子定位和状态。结果表明，Amb在聚乙二醇脂质体中的定位有两种类型，一种对应于膜表面的Amb与DSPE-PEG的络合物，另一种对应于脂质体疏水核心中的Amb的孔形态。聚乙二醇脂质体中的AMB既有聚集状态，也有单体状态。
We investigated the molecular localization and state of amphotericin B (AmB) encapsulated in polyethylene glycol (PEG)-coated liposomes. AmB-encapsulating PEG-liposomes composed of dipalmitoylphosphatidylcholine (DPPC), cholesterol (CH) and distearoyl-N-(monomethoxy poly(ethylene glycol)succinyl) phosphatidylethanolamine (DSPE-PEG, average MW of the PEG chain 2000) were prepared by hydration with 9% sucrose solution and extrusion. The amount of AmB encapsulated in the liposomes increased with incorporation of DSPE-PEG and decreased with that of CH. The molecular localization and state of AmB were investigated by PEG/dextran two-phase partition, potassium permeability measurement, fluorescence quenching measurement and circular dichroism (CD) spectroscopy. The results suggest that there are two types of AmB localization in PEG-liposomes, one of which corresponds to the complex of AmB with DSPE-PEG on the membrane surface, while the other corresponds to the pore form of AmB in the hydrophobic core of the liposomal membrane. AmB in PEG liposomes was present in both aggregated and monomeric states.
两性霉素 B 选择性胆固醇依赖性诱导磷脂囊泡中的 H/OH-电流。
影响因子：
2.9
作者：
Hartsel,SC;Perkins,WR;McGarvey,GJ;Cafiso,DS
通讯作者：
Cafiso,DS