Effect of a novel fluorapatite-forming calcium phosphate cement with calcium silicate on osteoblasts in comparison with mineral trioxide aggregate.
Effect of a novel fluorapatite-forming calcium phosphate cement with calcium silicate on osteoblasts in comparison with mineral trioxide aggregate.
复制标题
与三氧化二矿物骨料相比，新型氟磷灰石形成磷酸钙水泥与硅酸钙对成骨细胞的影响。
作者：
Yusuke Suzuki;Makoto Hayashi;N. Tanabe;Takuya Yasukawa;Y. Hirano;S. Takagi;Laurence C. Chow;N. Suzuki;B. Ogiso
我们比较了含硅酸三钙（TCS）的氟磷灰石形成磷酸钙骨水泥（FA形成CPC）和三氧化矿物聚集体（MTA）（金标准牙髓骨水泥）对培养的成骨细胞样细胞（ROS 17/2.8细胞; ROS细胞）的治疗效果。形成FA的CPC粉末由61.29%CaHPO4、32.26%CaCO3和6.45%NaF组成。将一份TCS与九份FA成型CPC粉末组合以制备具有TCS的FA成型CPC。将1.5M磷酸盐溶液以2.22的粉/液比混合作为骨水泥液体。使用细胞培养插入物进行细胞培养，其中将每种试验材料置于细胞培养板中的多孔膜插入物上。在形成FA的CPC与TCS和MTA的存在下，测量ROS细胞的增殖、形态学变化和碱性磷酸酶活性并进行比较。ROS细胞的对数生长期和细胞形态学变化在所有实验组中是相同的。此外，在暴露于FA形成CPC与TCS和暴露于MTA的ROS细胞中，碱性磷酸酶活性没有显著差异。总之，FA形成CPC与TCS具有相同的特性，MTA在本实验条件下，因此可能是有用的牙髓应用。
We compared the effects of treatment with fluorapatite-forming calcium phosphate cement (FA-forming CPC) containing tricalcium silicate (TCS) and those of mineral trioxide aggregate (MTA), the gold standard endodontic cement, on cultured osteoblast-like cells (ROS 17/2.8 cells; ROS cells). The FA-forming CPC powder consisted of 61.29% CaHPO4, 32.26% CaCO3, and 6.45% NaF. One part TCS was combined with nine parts FA-forming CPC powder to make FA-forming CPC with TCS. A 1.5-M phosphate solution was mixed as a cement liquid with a powder/liquid ratio of 2.22. Cell culture was carried out using cell culture inserts, whereby each test material was put on a porous membrane insert in the cell culture plate. Proliferation, morphologic changes, and alkaline phosphatase activity in ROS cells were measured in the presence of FA-forming CPC with TCS and MTA and compared. The logarithmic growth phase and cellular morphologic changes in ROS cells were identical in all experimental groups. Additionally, no significant difference in alkaline phosphatase activity was noted in ROS cells exposed to FA-forming CPC with TCS and those exposed to MTA. In conclusion, FA-forming CPC with TCS has characteristics identical to those of MTA under the present experimental conditions and may thus be useful for endodontic applications.
氟磷灰石增强间充质/内皮共培养物的矿化。
影响因子：
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作者：
Wang,Xiaodong;Zhang,Zhaocheng;Chang,Syweren;Czajka-Jakubowska,Agata;Nör,JacquesE;Clarkson,BrianH;Ni,Longxing;Liu,Jun
通讯作者：
Liu,Jun