Fluorapatite enhances mineralization of mesenchymal/endothelial cocultures.
Fluorapatite enhances mineralization of mesenchymal/endothelial cocultures.
复制标题
氟磷灰石增强间充质/内皮共培养物的矿化。
DOI：
10.1089/ten.tea.2013.0113
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发表时间：
2014
影响因子：
--
通讯作者：
Liu,Jun
中科院分区：
文献类型：
--
作者：
Wang,Xiaodong;Zhang,Zhaocheng;Chang,Syweren;Czajka-Jakubowska,Agata;Nör,JacquesE;Clarkson,BrianH;Ni,Longxing;Liu,Jun
除了广泛使用的间充质干细胞(MSCs)外，内皮细胞似乎也是硬组织再生的有利细胞来源。此前，氟磷灰石被证明可以刺激和增强MSCs的矿化。本研究旨在研究内皮细胞在合成的有序氟磷灰石表面的生长及其对脂肪干细胞(ASCs)共培养矿化的影响。在氟磷灰石表面培养内皮细胞，并用细胞计数、流式细胞仪、扫描电子显微镜和酶联免疫吸附试验(EL ISA)对其进行表征。细胞与ASCs共培养，碱性磷酸酶染色和矿物质形成。通过对成纤维细胞生长因子途径的干扰和碱性成纤维细胞生长因子的处理，观察其对ASCs分化和矿化的影响。氟磷灰石表面对内皮细胞具有良好的生物相容性。在没有矿化补充的情况下，与内皮细胞共培养诱导ASCs成骨分化，氟磷灰石表面进一步促进了ASCs的成骨分化。这表明内皮细胞和氟磷灰石表面对ASCs矿化的促进作用是联合作用的。经氟磷灰石表面刺激的ASCs单独或与ASCs共培养时，内皮细胞释放更多的bFGF，再加上对血管内皮细胞生长因子通路的干扰和对碱性成纤维细胞生长因子的处理，提示成纤维细胞生长因子信号通路可能在这一过程中起作用。
In addition to the widely used mesenchymal stem cells (MSCs), endothelial cells appear to be a favorable cell source for hard tissue regeneration. Previously, fluorapatite was shown to stimulate and enhance mineralization of MSCs. This study aims to investigate the growth of endothelial cells on synthesized ordered fluorapatite surfaces and their effect on the mineralization of adipose-derived stem cells (ASCs) through coculture. Endothelial cells were grown on fluorapatite surfaces and characterized by cell counting, flow cytometry, scanning electron microscopy, and enzyme-linked immunosorbent assay (ELISA). Cells were then cocultured with ASCs and stained for alkaline phosphatase and mineral formation. Fibroblast growth factor (FGF) pathway perturbation and basic FGF (bFGF) treatment of the ASCs were also conducted to observe their effects on differentiation and mineralization of these cells. Fluorapatite surfaces showed good biocompatibility in supporting endothelial cells. Without a mineralization supplement, coculture with endothelial cells induced osteogenic differentiation of ASCs, which was further enhanced by the fluorapatite surfaces. This suggested a combined stimulating effect of endothelial cells and fluorapatite surfaces on the enhanced mineralization of ASCs. Greater amounts of bFGF release by endothelial cells alone or cocultures with ASCs stimulated by fluorapatite surfaces, together with FGF pathway perturbation and bFGF treatment results, suggested that the FGF signaling pathway may function in this process.
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