Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia
Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia
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柬埔寨一种先前未描述的单部分 Begomovirus 和感染 Malvastrum coromandelianum 的 β 卫星的完整基因组序列
DOI：
10.1007/s00705-021-05016-y
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发表时间：
2021-04
影响因子：
2.7
通讯作者：
何自福
中科院分区：
文献类型：
--
作者：
汤亚飞;李正刚;佘小漫;于琳;蓝国兵;吕利华;Judith K. Brown;何自福
在柬埔寨贡布省发现了一种以前未被描述的单分部大麦病毒，该病毒在表现出大麦病毒感染特征的黄脉症状的冠果锦葵植株中被发现。采用滚环扩增和限制性内切酶双酶切富集环状DNA，克隆并测序了该病毒的全长片段。该病毒基因组全长2737个核苷酸(KP188831)，具有与其他单部分秋海棠病毒相似的组织结构，与刺参黄脉病毒(AM940137)的核苷酸序列相似性最高(87.7%)。利用卫星特异引物对β01/β02进行聚合酶链式反应，扩增出卫星分子。该卫星分子(1346nt，KP188832)与苹果黄脉卫星(MN205547)的核苷酸序列同源性最高(84.8%)，具有与秋病毒相关的其他卫星的结构特征。根据双生病毒科(Geminiviridae)和贝氏卫星病毒(Betasatellitiddae)的种划分标准，柬埔寨冠脉支原体(M.coromandelianum)的病毒分离物是一种以前未被描述的新的单子组病毒，建议将其命名为“苹果星黄脉柬埔寨病毒”(Malvstar um Huang vein柬埔寨Virus，MaYVCV)，而β卫星是一种以前未描述的新的β卫星，建议将其命名为“苹果星黄脉柬埔寨贝塔卫星”(MaYVKHB)。
A previously undescribed monopartite begomovirus was identified in Kampot province, Cambodia, in Malvastrum coromandelianum plants exhibiting yellow vein symptoms characteristic of begomovirus infections. The apparently full-length viral component was cloned and sequenced following enrichment of circular DNA by rolling-circle amplification and restriction enzyme digestion. The genome of the virus was 2737 nucleotides in length (KP188831) and exhibited an organization like that of other monopartite begomoviruses, sharing the highest nucleotide sequence similarity (87.7% identity) with ageratum yellow vein virus (AM940137). A satellite molecule was amplified from total DNA by PCR amplification, using the betasatellite-specific primer pair β01/β02. The satellite molecule (1346 nt, KP188832) had structural characteristics like those of other betasatellites associated with begomoviruses and shared the highest nucleotide sequence similarity (84.8% identity) with malvastrum yellow vein betasatellite (MN205547). According to the criteria established for species demarcation for classification of begomoviruses (family Geminiviridae) and betasatellites (family Tolecusatellitidae), respectively, the virus isolate from M. coromandelianum in Cambodia is a previously undescribed novel monopartite begomovirus, for which the name “malvastrum yellow vein Cambodia virus” (MaYVCV) is proposed, and the betasatellite is a previously undescribed novel betasatellite, for which the name “malvastrum yellow vein Cambodia betasatellite” (MaYVKHB) is proposed.
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影响因子：
2.7
作者：
A. Varsani;bullet J Jesú;Navas-Castillo bullet;E. Moriones;C. Hernández-Zepeda;bullet Ali;Idris bullet-I
通讯作者：
A. Varsani;bullet J Jesú;Navas-Castillo bullet;E. Moriones;C. Hernández-Zepeda;bullet Ali;Idris bullet-I
影响因子：
0.9
作者：
S. U. Rahman;M. Zubair;N. Ahmed;M. Z. Khan;G. Raza;I. Amin;S. Mansoor
通讯作者：
S. U. Rahman;M. Zubair;N. Ahmed;M. Z. Khan;G. Raza;I. Amin;S. Mansoor
影响因子：
5.3
作者：
Martin DP;Murrell B;Golden M;Khoosal A;Muhire B
通讯作者：
Muhire B
影响因子：
10.2
作者：
Xue-ping Zhou
通讯作者：
Xue-ping Zhou
影响因子：
2.7
作者：
W. N. Leke;A. Kvarnheden;S. Avelar;Judith K. Brown
通讯作者：
W. N. Leke;A. Kvarnheden;S. Avelar;Judith K. Brown