Light regulation of plant gene expression by an upstream enhancer-like element
Light regulation of plant gene expression by an upstream enhancer-like element
复制标题
上游类增强子元件对植物基因表达的光调节
作者：
Michael P. Timko;A. Kausch;C. Castresana;Jan S. Fassler;L. Herrera;G. Broeck;M. Montagu;J. Schell;A. Cashmore
在植物生长和分化过程中，光调节许多不同的生理和发育现象，包括由原质体形成具有光合能力的叶绿体1。核酮糖1，5-二磷酸羧化酶小亚基（rbcS）基因的表达受光的调节，在发育和组织特异性的方式2，3。在一些植物物种中，光敏色素已被证明介导这种反应4 -7，并且rbcS表达的光调节至少部分发生在转录水平8，9。我们以前已经证明豌豆rbcS ss3.6基因的5′-非编码片段（信使RNA帽位点上游4-973个碱基对（bp））包含指导烟草中细菌氯霉素乙酰转移酶（cat）基因光调控表达所需的所有核苷酸序列信息10。与这些发现一致，Morelli等11通过对第二个rbcS基因启动子的缺失分析表明，rbcS E9光调控表达所需的序列位于5′-非编码区。他们确定了最大转录所需的约700 bp的上游区域，但不是光暗调节所需的，以及从-35到-2 bp的区域，其中包括TATA盒，并包含光响应的必要信息。我们现在证明，调控序列5′远端的rbcS ss3.6 TATA框和转录起始位点不仅包含必要的信息，最大的表达，而且赋予光调节。这些上游调控序列在与它们的同源启动子或异源启动子融合时独立于方向发挥功能。
Light regulates many varied physiological and developmental phenomena during plant growth and differentiation, including the formation of a photosynthetically competent chloroplast from a proplastid1. The expression of ribulose 1,5-bisphosphate carboxylase small subunit (rbcS) genes is regulated by light in a development- and tissue-specific manner2,3. In some plant species, phytochrome has been demonstrated to mediate this response4–7, and photoregulation of rbcS expression occurs at least in part at the level of transcription8,9. We have shown previously that a 5′-noncoding fragment (4–973 base pairs (bp) upstream of the messenger RNA cap site) of the pea rbcS ss3.6 gene contains all of the nucleotide sequence information necessary to direct the photoregulated expression of a bacterial chloramphenicol acetyltransferase (cat) gene in tobacco10. Consistent with these findings, Morelli et al.11 have shown by deletion analysis of a second rbcS gene promoter, that the sequences required for photo-regulated expression of rbcS E9 reside within the 5′-noncoding region. They identified an upstream region of ∼700 bp needed for maximum transcription but not light–dark regulation, and a region from −35 to −2 bp which included the TATA box and contained the necessary information for light responsiveness. We now demonstrate that regulatory sequences 5′ distal to the rbcS ss3.6 TATA box and transcriptional start site not only contain the information necessary for maximum expression, but also confer photoregulation. These upstream regulatory sequences function independently of orientation when fused to their homologous promoter or a heterologous promoter.
DOI：
10.1073/pnas.81.24.7860
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GUARENTE, L;HOAR, E
通讯作者：
HOAR, E
酵母半乳糖调节元件的遗传特性和染色质结构：增强子样序列。
DOI：
10.1073/pnas.81.24.7865
发表时间：
1984
影响因子：
11.1
作者：
Struhl,K
通讯作者：
Struhl,K