Bifidobacterium longum alleviates irritable bowel syndrome-related visceral hypersensitivity and microbiota dysbiosis via Paneth cell regulation.
Bifidobacterium longum alleviates irritable bowel syndrome-related visceral hypersensitivity and microbiota dysbiosis via Paneth cell regulation.
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长双歧杆菌通过潘氏细胞调节减轻肠易激综合征相关的内脏过敏和微生物群失调
DOI：
10.1080/19490976.2020.1782156
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发表时间：
2020-11-09
期刊：
影响因子：
12.2
通讯作者：
Zou D
中科院分区：
文献类型：
--
作者：
Zhou C;Fang X;Xu J;Gao J;Zhang L;Zhao J;Meng Y;Zhou W;Han X;Bai Y;Li Z;Zou D
虽然口服长双歧杆菌（B. longum）在临床环境中减轻肠易激综合征（IBS）的体征，其作用的机制尚不清楚。在这项研究中，我们评估了B的精确效果。longum通过调节潘氏细胞功能对IBS的影响。我们证实了B的有益作用。对WAS大鼠排便习惯和内脏高敏感性的影响。进一步分析表明，B.在WAS大鼠中，longum增强粘膜修复，促进溶菌酶产生，并改善微生物群的生态失调。这些过程与潘氏细胞功能密切相关。在体外，我们用B孵育原代培养的肠上皮细胞。longum，发现B. longum促进了这些类器官的增殖;这可能归因于潘氏细胞分泌的干生态位因子WNT 3A和TGF-β的上调。根据我们的研究结果，我们建议，B。longum通过恢复潘氏细胞的抗微生物活性和干生态位维持功能来缓解IBS。
Although the oral administration of Bifidobacterium longum (B. longum) relieves the signs of irritable bowel syndrome (IBS) in clinical settings, the mechanisms underlying its effects are unclear. In this study, we evaluated the precise effects of B. longum on IBS via regulation of Paneth cell function. We confirmed the beneficial effects of B. longum on defecation habits and visceral hypersensitivity in WAS rats. Further analysis revealed that B. longum enhanced mucosal repair, promoted lysozyme production, and ameliorated dysbiosis of the microbiota in WAS rats. These processes are closely correlated with Paneth cell functions. In vitro, we incubated primary cultured enteroids with B. longum and found that B. longum promoted the proliferation of these organoids; this may be attributed to the upregulation of the stem niche factors WNT3A and TGF-β, which are secreted by Paneth cells. Based on our findings, we propose that B. longum relieves IBS by restoring the antimicrobial activity and stem niche maintenance function of Paneth cells.
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影响因子：
4.7
作者：
Moloney RD;O'Mahony SM;Dinan TG;Cryan JF
通讯作者：
Cryan JF
影响因子：
24.5
作者：
Cani PD;Possemiers S;Van de Wiele T;Guiot Y;Everard A;Rottier O;Geurts L;Naslain D;Neyrinck A;Lambert DM;Muccioli GG;Delzenne NM
通讯作者：
Delzenne NM
影响因子：
15.3
作者：
Menard, Sandrine;Foerster, Valentina;Lotz, Michael;Guetle, Dominique;Duerr, Claudia U.;Gallo, Richard L.;Henriques-Normark, Birgitta;Puetsep, Katrin;Andersson, Mats;Glocker, Erik O.;Hornef, Mathias W.
通讯作者：
Hornef, Mathias W.
影响因子：
6.8
作者：
Allen AP;Hutch W;Borre YE;Kennedy PJ;Temko A;Boylan G;Murphy E;Cryan JF;Dinan TG;Clarke G
通讯作者：
Clarke G
DOI：
10.1152/ajpgi.00500.2004
发表时间：
2005-07-01
影响因子：
4.5
作者：
Bradesi, S;Schwetz, I;Mayer, EA
通讯作者：
Mayer, EA