Innate Immune Interference Attenuates Inflammation In Bacillus Endophthalmitis.
Innate Immune Interference Attenuates Inflammation In Bacillus Endophthalmitis.
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DOI：
10.1167/iovs.61.13.17
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发表时间：
2020-11-02
影响因子：
4.4
通讯作者：
Callegan MC
中科院分区：
文献类型：
--
作者：
Mursalin MH;Coburn PS;Miller FC;Livingston ET;Astley R;Callegan MC
To explore the consequences of innate interference on intraocular inflammatory responses during Bacillus endophthalmitis. Bacillus endophthalmitis was induced in mice. Innate immune pathway activation was interfered by injecting S layer protein-deficient (∆slpA) B. thuringiensis or by treating wild-type (WT)–infected mice with a TLR2/4 inhibitor (WT+OxPAPC). At 10 hours postinfection, eyes were harvested and RNA was purified. A NanoString murine inflammation panel was used to compare gene expression in WT-infected, WT+OxPAPC, ∆slpA-infected, and uninfected eyes. In WT-infected eyes, 56% of genes were significantly upregulated compared to uninfected controls. Compared to WT-infected eyes, the expression of 27% and 50% of genes were significantly reduced in WT+OxPAPC and ∆slpA-infected eyes, respectively. Expression of 61 genes that were upregulated in WT-infected eyes was decreased in WT+OxPAPC and ∆slpA-infected eyes. Innate interference resulted in blunted expression of complement factors (C3, Cfb, and C6) and several innate pathway genes (TLRs 2, 4, 6, and 8, MyD88, Nod2, Nlrp3, NF-κB, STAT3, RelA, RelB, and Ptgs2). Innate interference also reduced the expression of several inflammatory cytokines (CSF2, CSF3, IL-6, IL-1β, IL-1α, TNFα, IL-23α, TGFβ1, and IL-12β) and chemokines (CCL2, CCL3, and CXCLs 1, 2, 3, 5, 9, and 10). All of the aforementioned genes were significantly upregulated in WT-infected eyes. These results suggest that interfering with innate activation significantly reduced the intraocular inflammatory response in Bacillus endophthalmitis. This positive clinical outcome could be a strategy for anti-inflammatory therapy of an infection typically refractory to corticosteroid treatment.
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DOI：
10.4049/jimmunol.1002122
发表时间：
2011-05-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Duggan JM;You D;Cleaver JO;Larson DT;Garza RJ;Guzmán Pruneda FA;Tuvim MJ;Zhang J;Dickey BF;Evans SE
通讯作者：
Evans SE
影响因子：
2
作者：
Callegan, Michelle C.;Cochran, Daniel C.;Stroman, David W.
通讯作者：
Stroman, David W.
DOI：
10.1007/978-3-319-23603-2_3
发表时间：
2015-01-01
期刊：
PROKARYOTIC SYSTEMS BIOLOGY
影响因子：
--
作者：
Dufresne, Karine;Paradis-Bleau, Catherine
通讯作者：
Paradis-Bleau, Catherine
影响因子：
3.1
作者：
Callegan, MC;Booth, MC;Gilmore, MS
通讯作者：
Gilmore, MS
影响因子：
3.1
作者：
Callegan, MC;Cochran, DC;Lereclus, D
通讯作者：
Lereclus, D