Adult-Onset Transcriptomic Effects of Developmental Exposure to Benzene in Zebrafish (Danio rerio): Evaluating a Volatile Organic Compound of Concern.
Adult-Onset Transcriptomic Effects of Developmental Exposure to Benzene in Zebrafish (Danio rerio): Evaluating a Volatile Organic Compound of Concern.
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DOI：
10.3390/ijms242216212
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发表时间：
2023-11-11
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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城市环境受到人为挥发性有机化合物（VOC）混合物的影响。导致人类接触的VOC来源包括汽车尾气、工业排放和石油泄漏。高挥发性VOC苯与不良健康结果有关。然而，很少有研究集中在低水平的苯暴露在早期发展的敏感窗口期间的后期影响。胚胎发育过程中的转录组反应在等于或低于1 ppm的水平下具有潜在的长期后果，因此证明了对早期发育过程中暴露的成年斑马鱼进行分析的合理性。以前，我们确定了转录组的改变后，控制VOC暴露于0.1或1 ppm的苯在胚胎发育的前五天使用斑马鱼模型。在这项研究中，我们评估了成人发病的转录组反应，这种低水平苯胚胎发生暴露（n = 20/治疗）。我们确定了关键基因，包括col 1a 2和evi 5 b，在两种浓度下成年斑马鱼中差异表达。一些DEG在幼虫和成虫阶段重叠，特别是nfkbiaa，mecr和reep 1。观察到的转录组结果表明，变化具有剂量和性别依赖性，苯暴露对癌症结局、内分泌系统疾病、生殖成功、神经发育、神经系统疾病和相关途径的影响最大。由于分子途径在斑马鱼和哺乳动物之间高度保守，发育暴露的成年斑马鱼转录组学是深入了解VOCs对人类健康和疾病的长期影响的重要终点。
Urban environments are afflicted by mixtures of anthropogenic volatile organic compounds (VOCs). VOC sources that drive human exposure include vehicle exhaust, industrial emissions, and oil spillage. The highly volatile VOC benzene has been linked to adverse health outcomes. However, few studies have focused on the later-in-life effects of low-level benzene exposure during the susceptible window of early development. Transcriptomic responses during embryogenesis have potential long-term consequences at levels equal to or lower than 1 ppm, therefore justifying the analysis of adult zebrafish that were exposed during early development. Previously, we identified transcriptomic alteration following controlled VOC exposures to 0.1 or 1 ppm benzene during the first five days of embryogenesis using a zebrafish model. In this study, we evaluated the adult-onset transcriptomic responses to this low-level benzene embryogenesis exposure (n = 20/treatment). We identified key genes, including col1a2 and evi5b, that were differentially expressed in adult zebrafish in both concentrations. Some DEGs overlapped at the larval and adult stages, specifically nfkbiaa, mecr, and reep1. The observed transcriptomic results suggest dose- and sex-dependent changes, with the highest impact of benzene exposure to be on cancer outcomes, endocrine system disorders, reproductive success, neurodevelopment, neurological disease, and associated pathways. Due to molecular pathways being highly conserved between zebrafish and mammals, developmentally exposed adult zebrafish transcriptomics is an important endpoint for providing insight into the long term-effects of VOCs on human health and disease.
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DOI：
10.1038/s41436-020-01003-x
发表时间：
2021-03
期刊：
Genetics in medicine : official journal of the American College of Medical Genetics
影响因子：
--
作者：
Bertrand RE;Wang J;Xiong KH;Thangavel C;Qian X;Ba-Abbad R;Liang Q;Simões RT;Sampaio SAM;Carss KJ;Lucy Raymond F;Robson AG;Webster AR;Arno G;Porto FBO;Chen R
通讯作者：
Chen R
影响因子：
7.8
作者：
Farrugia, Aaron J.;Rodriguez, Javier;Calvo, Fernando
通讯作者：
Calvo, Fernando
影响因子：
10.4
作者：
Rowe BL;Toccalino PL;Moran MJ;Zogorski JS;Price CV
通讯作者：
Price CV
影响因子：
4.5
作者：
Berube, Roxanne;Gauthier, Charles;Couture, Patrice
通讯作者：
Couture, Patrice
影响因子：
--
作者：
Bu, Peilong;Xiao, Yafei;Hu, Shaowen;Jiang, Xiaowei;Tan, Cong;Qiu, Mengyuan;Huang, Wanting;Li, Mengmeng;Li, Quanying;Qin, Changjiang
通讯作者：
Qin, Changjiang