Factors of Severity in Patients with COVID-19: Cytokine/Chemokine Concentrations, Viral Load, and Antibody Responses.
Factors of Severity in Patients with COVID-19: Cytokine/Chemokine Concentrations, Viral Load, and Antibody Responses.
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DOI：
10.4269/ajtmh.20-1110
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Kim SH
中科院分区：
文献类型：
--
作者：
Kwon JS;Kim JY;Kim MC;Park SY;Kim BN;Bae S;Cha HH;Jung J;Kim MJ;Lee MJ;Choi SH;Chung JW;Shin EC;Kim SH
COVID-19的严重程度由轻微至危重不等。然而，有限的数据已发表的详细动力学的病毒载量和宿主免疫反应在整个疾病过程中取决于疾病的严重程度。在这项研究中，我们综合分析了病毒载量，抗体反应，SARS冠状病毒2，细胞因子/趋化因子在疾病过程中，并确定了严重程度的相关因素。鼻咽（NP）和血浆标本从31名住院期间的COVID-19患者中获得。通过逆转录-PCR定量NP标本中的病毒RNA。采用ELISA和流式微球芯片检测血浆标本中的抗SARS-CoV-2抗体和细胞因子/趋化因子。COVID-19患者的病毒载量在疾病早期达到峰值，并持续下降。重症和危重症病例比无症状和轻型病例病毒载量更高，病毒脱落时间更长。而血浆IgG逐渐增加，并在住院期间维持，血浆IgM在症状发作后3周达到峰值并消失。重症和危重症患者的抗体应答稍有延迟，但较其他患者强。症状发作后5-10天高水平的干扰素（IFN）-α、IFN-γ诱导蛋白-10、IFN-γ诱导的单核因子和白细胞介素-6与COVID-19的严重程度相关。我们的数据表明，呼吸道中的高病毒载量以及症状发作后1至2周内细胞因子和趋化因子的过度产生与COVID-19的严重程度显著相关。
The severity of COVID-19 ranges from mild to critical diseases. However, limited data have been published on the detailed kinetics of viral load and host immune response throughout the disease course depending on disease severity. In this study, we comprehensively analyzed viral load, antibody responses to SARS-CoV-2, and cytokines/chemokines during the disease course, and identified the factors related to severity. Nasopharyngeal (NP) and plasma specimens were obtained from 31 patients with COVID-19 during hospitalization. Viral RNA in NP specimens was quantified by reverse transcription–PCR. Anti–SARS-CoV-2 antibodies and cytokines/chemokines in plasma specimens were analyzed by ELISA and cytometric bead array. The viral load in patients with COVID-19 peaked at the early stage of the disease and continuously decreased. Severe and critical cases showed higher viral load and prolonged viral shedding than asymptomatic and mild cases. Whereas plasma IgG was gradually increased and maintained during hospitalization, plasma IgM peaked at 3 weeks after symptom onset and dissipated. The antibody response in severe and critical cases was slightly delayed but stronger than those in others. High levels of interferon (IFN)-α, IFN-γ–induced protein-10, monokine induced by IFN-γ, and interleukin-6 at 5–10 days from symptom onset were associated with the severity of COVID-19. Our data indicate that high viral load in the respiratory tract and excessive production of cytokines and chemokines between 1 and 2 weeks from the symptom onset were significantly associated with the severity of COVID-19.
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DOI：
10.1016/j.jcv.2018.01.017
发表时间：
2018-04
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Kwon JS;Kim MC;Kim JY;Jeon NY;Ryu BH;Hong J;Kim MJ;Chong YP;Lee SO;Choi SH;Kim YS;Woo JH;Kim SH
通讯作者：
Kim SH
DOI：
10.1056/nejmoa2022926
发表时间：
2020-11-19
期刊：
The New England journal of medicine
影响因子：
--
作者：
RECOVERY Collaborative Group;Horby P;Mafham M;Linsell L;Bell JL;Staplin N;Emberson JR;Wiselka M;Ustianowski A;Elmahi E;Prudon B;Whitehouse T;Felton T;Williams J;Faccenda J;Underwood J;Baillie JK;Chappell LC;Faust SN;Jaki T;Jeffery K;Lim WS;Montgomery A;Rowan K;Tarning J;Watson JA;White NJ;Juszczak E;Haynes R;Landray MJ
通讯作者：
Landray MJ
DOI：
10.15557/pimr.2020.0003
发表时间：
2020-01-01
影响因子：
3.6
作者：
通讯作者：
--
影响因子：
24.8
作者：
Lee, Jeong Seok;Park, Seongwan;Shin, Eui-Cheol
通讯作者：
Shin, Eui-Cheol
影响因子：
15.9
作者：
Wang, Yanqun;Zhang, Lu;Zhao, Jincun
通讯作者：
Zhao, Jincun