Molecular characterization of Cryptosporidium isolates from high-excreting young dairy calves in dairy cattle herds in Western France
Molecular characterization of Cryptosporidium isolates from high-excreting young dairy calves in dairy cattle herds in Western France
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法国西部奶牛群高分泌乳牛犊中隐孢子虫分离物的分子特征
DOI：
10.1007/s00436-013-3520-2
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发表时间：
2013
影响因子：
2
通讯作者：
C. Paraud
中科院分区：
文献类型：
--
作者：
A. Rieux;C. Chartier;I. Pors;A. Delafosse;C. Paraud
对法国诺曼底地区30个牛群的92头腹泻和非腹泻牛群的隐孢子虫阳性粪便样品进行了检测。在这里，目的是调查7-17日龄奶牛体内排泄的隐孢子虫种类和微小隐孢子虫的亚型。排泄量为每克粪便2个 × 10~4个卵囊和4个 × ~7卵囊。用18S SSU rRNA套式聚合酶链式反应结合测序鉴定隐孢子虫的种类，除2只牛隐孢子虫感染动物外，大多数病例(80/82)均可检出微小隐孢子虫。然后，将微小隐孢子虫样本提交给gp60聚合酶链式反应。利用4个微卫星座位(MM19、MM5、MSF和MS9-Mallon)对来自24个不同群体的39个样本进行了多座位分析。将这些结果与gp60的序列分析相结合，得到多位点型(MLT)。在此，微小隐孢子虫gp60基因分型鉴定出IIa人畜共患病等位基因家族中的三种亚型：IIaA15G2R1(88%)、IIaA16G3R1(10%)和IIaA19G2R1(2%)，我们发现了12个MLT。MS9-Mallon基因座的遗传多样性最高(5个等位基因)。最常见的MLT是MLT 1，有10个农场的15个样本：(MS9-M：298，MSF：165，MM5：264，MM19：462，gp60亚型：IIaA15G2R1)。当比较腹泻和非腹泻粪便样本时，隐孢子虫种类、微小隐孢子虫gp60亚型和MLT的分布没有差异。这里，在腹泻型和非腹泻型犊牛的卵囊排泄量为104-107 OPG的范围内，感染主要是由微小弧菌和人畜共患亚型IIaA15G2R1引起的。
Ninety-two Cryptosporidium sp.-positive fecal samples of dairy diarrheic or non-diarrheic calves from 30 cattle herds in Normandy (France) were selected. Here, the aim was to investigate the species of Cryptosporidium excreted as well as the subtypes of Cryptosporidium parvum found in 7–17-day-old dairy calves. Excretion levels were comprised between 2 × 104 and 4 × 107 oocysts per gram of feces. Here, a nested 18S SSU rRNA PCR associated with sequencing was performed for identification of Cryptosporidium species and revealed the presence of C. parvum in most cases (80/82), except for two animals which were infected with Cryptosporidium bovis. Then, C. parvum samples were submitted to gp60 PCR. For 39 samples from 24 different herds, a multilocus analysis based on four mini-microsatellites loci (MM19, MM5, MSF, and MS9-Mallon) were conducted. These results were combined with sequence analysis of the gp60 to obtain multilocus types (MLTs). Here, C. parvum gp60 genotyping identified three subtypes in the IIa zoonotic allele family: IIaA15G2R1 (88 %), IIaA16G3R1 (10 %), and IIaA19G2R1 (2 %), and we identified 12 MLTs. The MS9-Mallon locus was reported as the most polymorphic (five alleles). The most common MLT was MLT 1 with 15 samples in 10 farms: (MS9-M: 298, MSF: 165, MM5: 264, MM19: 462, and gp60 subtype: IIaA15G2R1). When comparing diarrheic and non-diarrheic fecal samples, no difference was seen for distribution of Cryptosporidium species, C. parvum gp60 subtypes, and MLTs. Here, in a range of oocyst excretion of 104–107 opg, both in diarrheic and non-diarrheic calves, infection was mainly due to C. parvum and to the zoonotic subtype: IIaA15G2R1.
影响因子：
6.4
作者：
Xiao, LH;Bern, C;Lal, AA
通讯作者：
Lal, AA