Apparent cerebral cytochrome aa3 reduction during cardiopulmonary bypass in hypoxaemic children with congenital heart disease. A critical analysis of in vivo near-infrared spectrophotometric data.
Apparent cerebral cytochrome aa3 reduction during cardiopulmonary bypass in hypoxaemic children with congenital heart disease. A critical analysis of in vivo near-infrared spectrophotometric data.
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先天性心脏病低氧血症儿童体外循环期间脑细胞色素 aa3 明显减少。
DOI：
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发表时间：
1994
影响因子：
3.2
通讯作者：
G. Greisen
中科院分区：
文献类型：
--
作者：
L. Skov;G. Greisen
本研究旨在通过近红外分光光度法（NIRS）检测14例心脏直视手术患儿体外循环（CPB）诱导期间脑细胞色素氧化酶的变化。5名儿童在转为CPB前出现低氧血症（动脉血氧饱和度49%-84%），9名儿童没有。在低氧血症的儿童，总脑血红蛋白浓度[tHb]迅速显着下降，2分钟后达到平台期。令人惊讶的是，细胞色素aa 3浓度在其氧化形式[CytO 2]平行下降，而脑血红蛋白氧合指数[HbO 2]-[Hb]逐渐增加。在8名血氧正常的儿童中，NIRS信号的变化不显著。当使用标准NIRS算法时，[CytO 2]的变化幅度与[tHb]的变化幅度密切相关（p < 0.0001），14名儿童中有11名儿童的[CytO 2]和[tHb]信号的时程平行，NIRS多组分分析的拟合误差比系统噪声增加10至100倍。一种新的近红外光谱算法，使用波长特定的光程长，没有改善拟合误差，但产生较小的估计[CytO 2]的变化，这是无关的[tHb]的变化。在我们看来，通过近红外光谱监测细胞色素氧化的有趣的可能性需要进一步验证。
The purpose of this analysis was critically to examine the changes of cerebral cytochrome oxidase as detected by near-infrared spectrophotometry (NIRS) during induction of cardiopulmonary bypass (CPB) in fourteen children undergoing open heart surgery. Five children were hypoxaemic (arterial oxygen saturation 49%-84%) before the switch to CPB and nine children were not. In the hypoxaemic children, the total cerebral hemoglobin concentration [tHb] decreased rapidly and markedly, reaching a plateau after 2 min. Surprisingly, cytochrome aa3 concentration in its oxygenated form [CytO2] decreased in parallel while the cerebral haemoglobin oxygenation index [HbO2]-[Hb] increased gradually. In the eight normoxaemic children, changes in the NIRS signals were insignificant. When a standard NIRS algorithm was used, the magnitude of the change in [CytO2] was closely associated with the magnitude of the change in [tHb] (p < 0.0001), the time courses of the [CytO2] and [tHb] signals were parallel in 11 of the 14 children and the error of fit in the NIRS multicomponent analysis increased 10- to 100-fold over system noise. A new NIRS algorithm, using wavelength specific optical pathlengths, did not improve the error of fit but produced smaller estimates of [CytO2] changes, which were unrelated to the [tHb] changes. In our opinion the interesting possibility of monitoring cytochrome oxidation by NIRS requires further validation.
用于确定组织氧合的时间和频率分辨光谱的定量。
影响因子：
2.9
作者：
Sevick,EM;Chance,B;Leigh,J;Nioka,S;Maris,M
通讯作者：
Maris,M