Altered distribution of interstitial cells and innervation in the rat urinary bladder following spinal cord injury.
Altered distribution of interstitial cells and innervation in the rat urinary bladder following spinal cord injury.
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DOI：
10.1111/j.1582-4934.2011.01410.x
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发表时间：
2012-07
影响因子：
5.3
通讯作者：
McCloskey KD
中科院分区：
文献类型：
--
作者：
Johnston L;Cunningham RM;Young JS;Fry CH;McMurray G;Eccles R;McCloskey KD
据报道，间质细胞（IC）分布的变化与膀胱功能障碍有关。本研究调查了脊髓损伤（SCI）是否导致IC亚群（波形蛋白阳性，具有IC的超微结构特征）、膀胱壁内的平滑肌和神经的变化，以及细胞重塑与功能特性的相关性。脊髓损伤（T8/9横断）和假手术大鼠损伤后5周的膀胱用于离体压力-容积实验或处理的形态学分析与透射电子显微镜（TEM）和光/共聚焦显微镜。压力-容积关系显示SCI膀胱中的低压、超顺应性指示失代偿。广泛的波形蛋白阳性IC网络在假固有层和逼尿肌中是典型的，但在SCI后明显减少;半定量分析显示显着减少。抗神经丝蛋白和抗vAChT标记的神经在SCI后显著减少。透射电镜显示假手术组固有层IC和逼尿肌IC与含囊泡的神经曲张形成紧密的突触样接触。脊髓损伤后，固有层和逼尿肌IC在超微结构上受损，细胞突起回缩/丢失，并与细胞碎片和神经元降解区域相邻。平滑肌肥大是SCI组织常见的现象。总之，脊髓损伤后5周膀胱壁IC数量减少，神经支配减少，平滑肌肥大，顺应性增加。这些新的发现表明SCI后膀胱壁重塑影响平滑肌、神经和IC之间相互作用的完整性，并损害IC群体。IC减少和高顺应性表型之间的相关性表明，膀胱IC的破坏有助于支持功能障碍的SCI膀胱的病理生理过程。
Changes in the distribution of interstitial cells (IC) are reportedly associated with dysfunctional bladder. This study investigated whether spinal cord injury (SCI) resulted in changes to IC subpopulations (vimentin-positive with the ultrastructural profile of IC), smooth muscle and nerves within the bladder wall and correlated cellular remodelling with functional properties. Bladders from SCI (T8/9 transection) and sham-operated rats 5 weeks post-injury were used for ex vivo pressure–volume experiments or processed for morphological analysis with transmission electron microscopy (TEM) and light/confocal microscopy. Pressure-volume relationships revealed low-pressure, hypercompliance in SCI bladders indicative of decompensation. Extensive networks of vimentin-positive IC were typical in sham lamina propria and detrusor but were markedly reduced post-SCI; semi-quantitative analysis showed significant reduction. Nerves labelled with anti-neurofilament and anti-vAChT were notably decreased post-SCI. TEM revealed lamina propria IC and detrusor IC which formed close synaptic-like contacts with vesicle-containing nerve varicosities in shams. Lamina propria and detrusor IC were ultrastructurally damaged post-SCI with retracted/lost cell processes and were adjacent to areas of cellular debris and neuronal degradation. Smooth muscle hypertrophy was common to SCI tissues. In conclusion, IC populations in bladder wall were decreased 5 weeks post-SCI, accompanied with reduced innervation, smooth muscle hypertrophy and increased compliance. These novel findings indicate that bladder wall remodelling post-SCI affects the integrity of interactions between smooth muscle, nerves and IC, with compromised IC populations. Correlation between IC reduction and a hypercompliant phenotype suggests that disruption to bladder IC contribute to pathophysiological processes underpinning the dysfunctional SCI bladder.
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影响因子：
5.3
作者：
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通讯作者：
Junquera Escribano C
影响因子：
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作者：
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作者：
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通讯作者：
Brading, AF
影响因子：
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作者：
Drake, MJ;Hedlund, F;Brading, AF
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Brading, AF