High-resistance MDCK-C7 monolayers used for measuring invasive potency of tumour cells
High-resistance MDCK-C7 monolayers used for measuring invasive potency of tumour cells
复制标题
高阻MDCK-C7单层细胞用于测量肿瘤细胞的侵袭能力
DOI：
10.1007/s004240000282
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
H. Oberleithner
中科院分区：
文献类型：
--
作者：
J. Żak;S. Schneider;I. Eue;T. Ludwig;H. Oberleithner
抽象的。我们描述了一种电生理学方法，使用肾细胞作为体外测定系统来评估肿瘤细胞的内在侵袭能力。 Madin-Darby 犬肾细胞 (MDCK-C7) 的高电阻克隆在滤杯中生长至汇合。在市售电极室中测量 MDCK-C7 单层的跨上皮电阻。跨上皮电阻达到约 4,000 Ω cm2 后，人黑色素瘤或胰腺癌细胞与 MDCK-C7 单层共培养。两种癌细胞系都会在 24 小时或更晚后诱导耐药性破坏，具体取决于接种密度和细胞类型。 MDCK-C7 细胞基底外侧表面上的癌细胞接种引起 MDCK-C7 单层跨上皮电阻的类似降低。抵抗力破坏表明肿瘤细胞侵袭之前紧密连接的打开。总之，高电阻 MDCK-C7 细胞克隆可以作为一种有价值的生物检测系统，用于体外电学测定肿瘤细胞的转移能力。
Abstract. We describe an electrophysiological method for evaluating the intrinsic invasive potency of tumour cells using renal cells as an in vitro assay system. A high-resistance clone of Madin-Darby canine kidney cells (MDCK-C7) was grown to confluency in a filter cup. Transepithelial electrical resistance across the MDCK-C7 monolayer was measured in a commercially available electrode chamber. After a transepithelial electrical resistance of about 4,000 Ω cm2 had been reached, human melanoma or pancreatic carcinoma cells were co-cultivated with the MDCK-C7 monolayer. Both carcinoma cell lines induced resistance breakdown measured after 24 h or later depending on seeding density and cell type. Seeding carcinoma cells on the basolateral surface of MDCK-C7 cells caused a similar decrease in transepithelial resistance of the MDCK-C7 monolayer. Resistance breakdown indicates opening of tight junctions prior to tumour cell invasion. In conclusion, the high-resistance MDCK-C7 cell clone could serve as a valuable biological assay system to determine electrically the metastatic potency of tumour cells in vitro.
DOI：
10.1073/pnas.94.15.7959
发表时间：
1997-07-22
影响因子：
11.1
作者：
Nakahara, H;Howard, L;Chen, WT
通讯作者：
Chen, WT
影响因子：
56.9
作者：
CUNNINGHAM, CC;GORLIN, JB;STOSSEL, TP
通讯作者：
STOSSEL, TP