Expression-based identification of genetic determinants of the bacterial symbiosis 'Chlorochromatium aggregatum'.
Expression-based identification of genetic determinants of the bacterial symbiosis 'Chlorochromatium aggregatum'.
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基于表达的细菌共生“Chlorochromatiumaggregatum”遗传决定因素的鉴定
DOI：
10.1111/j.1462-2920.2010.02206.x
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发表时间：
2010
影响因子：
5.1
通讯作者：
Overmann
中科院分区：
文献类型：
--
作者：
Wenter;Dibbern;Reisinger;Plöscher;Eichacker;Hanson;Bryant;Overmann
光合共生体“Chlorochromatium aggregatum”是一种高度结构化的绿色硫细菌外生体围绕一个中心，能动的细菌，是目前已知的最具体的共生体之间的两个共生遗传不同的细菌物种。在基因组、转录组和蛋白质组水平上鉴定了可能参与共生相互作用的基因和基因产物。与11个可获得的自由生活亲属的基因组相比，只有186个开放阅读框架被发现是表生体基因组所独有的。可溶性蛋白质组的2-D差异凝胶电泳（2-D DIGE）回收了1612个蛋白质点，其中54个仅在聚生体中检测到，而在纯的表生体培养物中未检测到。使用质谱分析，54个斑点中最强烈的13个可以归因于附着生物。对聚生体、用交联剂处理的聚生体和纯培养物的膜蛋白的分析表明，支链氨基酸ABC转运蛋白结合蛋白仅在附着生物的共生状态下表达。此外，叶绿体的分析表明，一个未知的11 kDa的表生体蛋白只在共生过程中表达。 该蛋白可能参与叶绿体的细胞内分选。一种新的原核cDNA抑制消减杂交技术的应用导致14个差异调节基因的鉴定，共生和自由生活的表生体的转录组的比较表明，328个基因的差异转录。这三种方法基本上是互补的，因此产生了352个基因的第一份清单，这些基因可能参与了C. aggregatum“。值得注意的是，大多数受调控的基因编码的组件的中央代谢途径，而只有很少（7.5%）的独特的“共生基因”被证明是受调控的实验条件下测试。这种对中枢代谢途径的明显调节可能有助于微调'C'中的共生相互作用。aggregatum“的反应环境条件。
The phototrophic consortium ‘Chlorochromatium aggregatum’ is a highly structured association of green sulfur bacterial epibionts surrounding a central, motile bacterium and is the most specific symbiosis currently known between two phylogenetically distinct bacterial species. Genes and gene products potentially involved in the symbiotic interaction were identified on the genomic, transcriptomic and proteomic level. As compared with the 11 available genomes of free‐living relatives, only 186 open reading frames were found to be unique to the epibiont genome. 2‐D differential gel electrophoresis (2‐D DIGE) of the soluble proteomes recovered 1612 protein spots of which 54 were detected exclusively in consortia but not in pure epibiont cultures. Using mass spectrometry analyses, the 13 most intense of the 54 spots could be attributed to the epibiont. Analyses of the membrane proteins of consortia, of consortia treated with cross‐linkers and of pure cultures indicated that a branched chain amino acid ABC‐transporter binding protein is only expressed in the symbiotic state of the epibiont. Furthermore, analyses of chlorosomes revealed that an uncharacterized 11 kDa epibiont protein is only expressed during symbiosis. This protein may be involved in the intracellular sorting of chlorosomes. Application of a novel prokaryotic cDNA suppression subtractive hybridization technique led to identification of 14 differentially regulated genes, and comparison of the transcriptomes of symbiotic and free‐living epibionts indicated that 328 genes were differentially transcribed. The three approaches were mostly complementary and thereby yielded a first inventory of 352 genes that are likely to be involved in the bacterial interaction in ‘C. aggregatum’. Notably, most of the regulated genes encoded components of central metabolic pathways whereas only very few (7.5%) of the unique ‘symbiosis genes’ turned out to be regulated under the experimental conditions tested. This pronounced regulation of central metabolic pathways may serve to fine‐tune the symbiotic interaction in ‘C. aggregatum’ in response to environmental conditions.
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高度发达的细菌共生体“Chlorochromatiumaggregatum”中四个候选共生基因的鉴定和分析
影响因子：
5.1
作者：
Wenter;Dressen;Schlickenrieder;Plöscher;Eichacker;Overmann
通讯作者：
Overmann
影响因子：
56.9
作者：
Reidun Sirevag;John G. Ormerod
通讯作者：
John G. Ormerod
影响因子：
3.4
作者：
Granvogl, Bernhard;Gruber, Patrick;Eichacker, Lutz Andreas
通讯作者：
Eichacker, Lutz Andreas
影响因子：
2.8
作者：
Dalisay, Doralyn S.;Webb, Jeremy S.;Kjelleberg, Staffan
通讯作者：
Kjelleberg, Staffan
DOI：
10.1073/pnas.86.8.2637
发表时间：
1989-04-01
影响因子：
11.1
作者：
RELMAN, DA;DOMENIGHINI, M;FALKOW, S
通讯作者：
FALKOW, S