Expression of α- and β-tubulin genes during development of sea urchin embryos☆
Expression of α- and β-tubulin genes during development of sea urchin embryos☆
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海胆胚胎发育过程中α-和β-微管蛋白基因的表达☆
DOI：
10.1016/0012-1606(85)90470-1
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发表时间：
1985
影响因子：
2.7
通讯作者：
J. Ruderman
中科院分区：
文献类型：
--
作者：
D. Alexandraki;J. Ruderman
海胆（Lytechinus pictus）成熟的未受精卵中含有多个α-微管蛋白mRNA，大小在1.75 ~ 4.8 kb之间，以及两个β-微管蛋白mRNA，大小分别为1.8和2.25 kb。这些mRNA在整个早期卵裂阶段以相似的水平被发现。RNA凝胶印迹杂交结果表明，微管蛋白mRNA的显着的定量和定性变化发生在早期囊胚和孵化囊胚阶段。α-和β-微管蛋白mRNA的总量在囊胚和pluteus之间增加了2 - 5倍。这些增加主要是由于1.75-kb的α RNA和新的2.0-kb的β RNA的增加。在随后的开发过程中，也发生了其他微小的变化。在早期和晚期胚胎中，α-和β-微管蛋白RNA的所有大小类别都含有poly（A）+可翻译序列。如前所述，一些α RNA在卵子中被翻译，但β RNA都不被翻译，并且在受精后30分钟内，储存的α和β RNA中的一小部分被募集到多核糖体上。在这里描述的工作中，随后的发展到桑椹胚阶段伴随着微管蛋白mRNA逐渐招募到多核糖体中。到早期囊胚阶段，大多数母体微管蛋白序列与多聚核糖体相关。与整个卵裂过程中母体序列的gradula募集相反，在囊胚阶段出现的微管蛋白mRNA在进入多聚核糖体中没有延迟。在后期阶段具有抑制活性的每种mRNA的确切分数在个体mRNA中略有不同。从卵、胚胎和睾丸RNA与各种微管蛋白cDNA和基因组DNA探针的差异杂交模式可以得出结论，至少有一个产生母体α mRNA的基因不同于仅在睾丸中表达的第二个基因。三种胚胎β RNA中的每一种都由不同的β基因编码;这些不同的β基因中至少有两种也在睾丸中表达。
Mature unfertilized eggs of the sea urchinLytechinus pictuscontain multiple α-tubulin mRNAs, which range in size from 1.75 to 4.8 kb, and two β-tubulin mRNAs, 1.8 and 2.25 kb. These mRNAs were found at similar levels throughout the early cleavage stages. RNA gel blot hybridizations showed that prominent quantitative and qualitative changes in tubulin mRNAs occurred between the early blastula and hatched blastula stages. The overall amounts of α- and β-tubulin mRNAs increased two- to fivefold between blastula and pluteus. These increases were due mainly to a rise in a 1.75-kb α RNA and a new 2.0-kb β RNA. Other, minor changes also occurred during subsequent development. All size classes of α- and β-tubulin RNAs in early and late embryos contained poly(A)+translatable sequences. As reported earlier, some of each of the α RNAs, but neither of the β RNAs, are translated in the egg and a small portion of each of the stored α and β RNAs is recruited onto polysomes within 30 min of fertilization. In the work described here, subsequent development up to the morula stage was accompanied by a gradual recruitment of tubulin mRNAs into polysomes. By the early blastula stage, most of the maternal tubulin sequences were associated with polysomes. In contrast to the gradula recruitment of maternal sequences throughout cleavage, the tubulin mRNAs which appeared at the blastula stage showed no delay in entering polysomes. The exact fraction of each mRNA that was translationally active at later stages varied somewhat among the individual mRNAs. From the differential hybridization patterns of egg, embryo, and testis RNAs to various tubulin cDNA and genomic DNA probes, it is concluded that at least one gene producing maternal α mRNA is different from a second one which is expressed only in testis. Each of the three embryonic β RNAs is encoded by a different β gene; at least two of these different β genes are also expressed in testis.
未受精海胆卵微管蛋白在生理温度下的组装。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Suprenant,KA;Rebhun,LI
通讯作者：
Rebhun,LI
海胆胚胎的分子生物学。
DOI：
10.1126/science.6178156
发表时间：
1982
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Davidson,EH;Hough-Evans,BR;Britten,RJ
通讯作者：
Britten,RJ