Study on immortal conditions of chicken embryonic stem cells
Study on immortal conditions of chicken embryonic stem cells
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鸡胚胎干细胞永生条件的研究
DOI：
10.1002/jcb.27173
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
Bichun Li
中科院分区：
文献类型：
--
作者：
Changhua Sun;Yingjie Wang;Kai Jin;Jiuzhou Song;Qisheng Zuo;Yani Zhang;Guohong Chen;Bichun Li
鸡胚胎干细胞（embryonic stem cells，ESCs）在基因治疗和再生医学中的广泛应用，近年来引起了人们的广泛关注。然而，所使用的方法仍不成熟。在这项研究中，我们发现具有清晰边界的鸡胚胎干细胞克隆可以稳定表达碱性磷酸酶和标记蛋白，如SSEA-1，SOX 2和OCT 4。另外，含10 μmol/L维生素C（VC）的培养液能显著促进ESCs的增殖。转染p：增强型绿色荧光蛋白（pEGFP）-hTERT的ESCs在含有外源因子（mLIF + bFGF + hSCF）和VC的培养基中可传代10代以上，冻存后仍能再生。定量真实的-时间聚合酶链反应结果显示，在ESC永生化过程中，SSEA-1、SOX 2和OCT 4表达之间没有显著差异，并且第十代ESC仍然能够表达标记蛋白SSEA-1、SOX 2和OCT 4。结果表明，我们建立了一种固定化胚胎干细胞的系统，并在体外培养的胚胎干细胞保持其多能性。
In recent years, considerable attention has been paid to chicken embryonic stem cells (ESCs) studies in relation to extensive applications in gene therapy and regenerative medicine. However, the approaches used are still immature. In this study, we showed that the chicken ESCs clones with a clear border can express alkaline phosphatase and marker proteins such as SSEA‐1, SOX2, and OCT4 stably. In addition, culture medium containing 10 μmol/L of vitamin C (VC) could significantly promote the proliferation of ESCs cells. Moreover, ESCs transfected with p:enhanced green fluorescent protein (pEGFP)‐hTERT could be subcultured more than tenth generations in culture medium containing exogenous factors (mLIF + bFGF + hSCF) and VC, and these ESCs clone could still be regenerated following cryopreservation. Quantitative real‐time polymerase chain reaction results showed that there was no significant difference between SSEA‐1, SOX2, and OCT4 expression during ESCs immortalization and that the tenth generation of ESCs was still able to express marker proteins SSEA‐1, SOX2, and OCT4. Our results showed that an immobilized system for ESCs was established, and the ESCs were cultured in vitro maintaining their pluripotency.
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DOI：
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发表时间：
2012
期刊：
--
影响因子：
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作者：
X. Min
通讯作者：
X. Min
影响因子：
4.4
作者：
Delany, ME;Daniels, LM;Taylor, HA
通讯作者：
Taylor, HA
DOI：
10.1007/978-1-62703-628-3_11
发表时间：
2013-01-01
期刊：
EPIBLAST STEM CELLS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Aubel, Pauline;Pain, Bertrand
通讯作者：
Pain, Bertrand
DOI：
--
发表时间：
2010
期刊：
Chinese journal of veterinary science
影响因子：
--
作者：
Wu Qing-xia;Wang Aihua;Si YongJia;Zhou Limei;Sheng HongXia;Jin Ya-ping
通讯作者：
Wu Qing-xia;Wang Aihua;Si YongJia;Zhou Limei;Sheng HongXia;Jin Ya-ping
水牛大鼠肝 3A 细胞 (BRL-3A) 条件培养基中鸡胚胎干细胞的培养
影响因子：
--
作者：
Geng, Li-Ying;Zhang, Chuan-Sheng;Ping, Shen;Du, Li-Xin;Liu, Zheng-Zhu;Fu, Zhi-Xin;Juan, Chen;Jia, Qing-Hui;Liu, Xie-Rong
通讯作者：
Liu, Xie-Rong