Metabonomics-based study of clinical urine samples in suboptimal health with different syndromes.
Metabonomics-based study of clinical urine samples in suboptimal health with different syndromes.
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DOI：
10.1155/2013/509134
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Chen JX
中科院分区：
文献类型：
--
作者：
Cui HZ;Wang LM;Zhao X;Liu YY;Wang SX;Li XH;Jiang YM;Chen JX
目标。探讨亚理想健康状态（SHS）下肝郁、脾虚、肝郁、脾虚证（LSSDS）中医证候的尿生化特征。方法：选取SHS各证候组12例，作为正常对照组12例，分别进行1H NMR检测，数据经正交信号校正（OSC）校正后，采用偏最小二乘法（PLS）判别分析。结果。核磁共振增强光谱（NOESY）检测的OSC-PLS （ctr）分析结果显示，SHS的证候是可以区分的，四组患者尿液中代谢物水平存在显著差异；发现了SHS中LSSDS的生物标志物。LSSDS组尿液中柠檬酸（2.54、2.66）、三甲胺氧化物（3.26）、马尿酸（3.98、7.54、7.58、7.62、7.66、7.82、7.86）含量均低于正常对照组。结论。四组尿液样品的1H-NMR代谢谱存在差异，通过代谢组学分析可以识别出SHS中LSSDS的特定代谢产物。
Objective. To explore the urinary biochemistry features of syndromes of traditional Chinese medicine (TCM) such as syndrome of stagnation of liver Qi, spleen deficiency, liver Qi stagnation, and spleen deficiency (LSSDS) in sub-optimal health status (SHS). Methods. 12 cases for each syndrome group in SHS were selected, 12 subjects were used as a normal control group, and 1H NMR detection was, respectively, carried out, and the data was corrected by the orthogonal signal correction (OSC) and then adopted a partial least squares (PLS) method for discriminate analysis. Results. The OSC-PLS (ctr) analysis results of the nuclear overhauser enhancement spectroscopy (NOESY) detection indicated that the syndromes in SHS could be differentiated, and there were significant differences in the levels of metabolites of the urine samples of the four groups; the biomarkers of LSSDS in SHS were found out. The contents of citric acid (2.54 and 2.66), trimethylamineoxide (3.26), and hippuric acid (3.98, 7.54, 7.58, 7.62, 7.66, 7.82, and 7.86) in the urine samples of LSSDS group were lower than that of the normal control group. Conclusion. There are differences in the 1H-NMR metabolic spectrum of the urine samples of the four groups, and the specific metabolic products of the LSSDS in SHS can be identified from metabonomics analysis.
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DOI：
10.1155/2012/489254
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Meng ZZ;Hu JH;Chen JX;Yue GX
通讯作者：
Yue GX
影响因子：
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DOI：
10.1016/j.jpba.2009.11.006
发表时间：
2010-04-06
影响因子：
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作者：
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Hashimoto, Takao
DOI：
10.1073/pnas.0605207103
发表时间：
2006-08-15
影响因子：
11.1
作者：
Ippolito, Joseph E.;Merritt, Matthew E.;Gordon, Jeffrey I.
通讯作者：
Gordon, Jeffrey I.