Xiaoyaosan decoction, a traditional chinese medicine, inhibits oxidative-stress-induced hippocampus neuron apoptosis in vitro.
Xiaoyaosan decoction, a traditional chinese medicine, inhibits oxidative-stress-induced hippocampus neuron apoptosis in vitro.
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DOI：
10.1155/2012/489254
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yue GX
中科院分区：
文献类型：
--
作者：
Meng ZZ;Hu JH;Chen JX;Yue GX
逍遥散汤是我国治疗精神障碍的名方。本实验探讨了XYS汤在体外逆转海马神经元凋亡的作用。我们使用XYS煎剂含药血清治疗氧化应激诱导的海马神经元凋亡，并使用免疫荧光法测定游离钙浓度、线粒体膜电位和神经元凋亡率。结果表明，3小时氧化应激通过引发细胞核碎裂、核固缩等细胞核病理改变，使线粒体膜电位降低，游离钙浓度升高，神经元凋亡率升高。低、中、高剂量的XYS煎剂含药血清均可逆转上述现象，且低剂量XYS煎剂含药血清对改善线粒体膜电位和神经元凋亡率有显着效果。这些结果表明，XYS 汤可能有助于减少氧化应激诱导的海马神经元凋亡。
Xiaoyaosan (XYS) decoction is a famous prescription for the treatment of mental disorders in China. In this experiment, we explored the way in which XYS decoction-reverse hippocampus neuron apoptosis in vitro. We used XYS decoction-containing serum to treat oxidative-stress-induced hippocampus neuron apoptosis and used immunofluorescence to determine the concentration of free calcium, mitochondrial membrane potential, and apoptotic rate of neuron. Results showed that 3-hour oxidative stress decrease mitochondrial membrane potential, increase the concentration of free calcium and apoptotic rate of neuron via triggering pathological changes of nucleus such as karyorrhexis, karyopyknosis. Low, medium, high dose of XYS-decoction-containing serum could reverse these phenomenon, and the effect of low-dose XYS-decoction-containing serum was significant in improving mitochondrial membrane potential and apoptotic rate of neuron. These findings suggest that XYS decoction may be helpful in reducing oxidative-stress-induced hippocampus neuron apoptosis.
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影响因子：
19
作者：
Green, D;Kroemer, G
通讯作者：
Kroemer, G
DOI：
10.1073/pnas.74.2.638
发表时间：
1977-01-01
影响因子：
11.1
作者：
KAISER, N;EDELMAN, IS
通讯作者：
EDELMAN, IS
影响因子：
6.1
作者：
Li, Lin;Zhang, Xuanhong;Tan, Hongmei
通讯作者：
Tan, Hongmei
影响因子：
5
作者：
Suzuki, K;Kostin, S;Schaper, J
通讯作者：
Schaper, J
影响因子：
2.5
作者：
Stanley, EF
通讯作者：
Stanley, EF