A planar on-chip micro-nib interface for NanoESI–MS microfluidic applications

A planar on-chip micro-nib interface for NanoESI–MS microfluidic applications
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用于 NanoESI-MS 微流体应用的平面片上微笔尖接口

DOI:
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发表时间:
2004
期刊:
影响因子:
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通讯作者:
C. Rolando
C. Rolando
中科院分区:
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文献类型:
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作者:
S. Arscott;S. Gac;C. Druon;P. Tabourier;C. Rolando

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我们提出了一种基于笔尖而不是喷嘴原理的新型纳米电喷雾发射器尖端,用于纳米电喷雾电离-质谱(ESI-MS)应用。微笔尖的制造依赖于使用环氧基负光刻胶SU-8的微加工技术。采用双曝光光刻工艺形成膜状结构的笔尖。笔尖端有一个20µm的毛细槽。nib源在离子阱质谱仪上使用低浓度(低至1 μ M)的标准肽样品成功地进行了测试。高压(HV)电源是通过在储液器中插入铂丝来实现的。从笔尖上可以清楚地看到一个泰勒锥,这是由毛细槽的尺寸决定的。
We present a novel nanoelectrospray emitter tip based on the principle of a nib rather than a nozzle for nano-electrospray ionization–mass spectrometry (ESI–MS) applications. The fabrication of the micro-nibs relies on micromachining techniques using the epoxy-based negative photoresist SU-8. A double exposure photolithographic process has been employed to form a nib in a membrane-like structure. The nibs contained a capillary slot measuring 20 µm at the tip end. The nib sources were successfully tested on an ion trap mass spectrometer using standard peptide samples at low concentrations, down to 1 µM. High voltage (HV) supply was achieved using platinum wire inserted in a liquid reservoir. A Taylor cone was clearly seen protruding from the nib tip and was determined by the dimensions of the capillary slot.
DOI: 10.1021/ac990967p
发表时间: 2000-01-15
影响因子: 7.4
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通讯作者: Lee, TD
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