Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers.
Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers.
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DOI：
10.3390/polym13223951
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发表时间：
2021-11-15
期刊：
影响因子：
5
通讯作者：
Varga G
中科院分区：
文献类型：
--
作者：
Földes A;Reider H;Varga A;Nagy KS;Perczel-Kovach K;Kis-Petik K;DenBesten P;Ballagi A;Varga G
牙髓外胚间充质干细胞来源于神经嵴，是细胞治疗和组织工程的重要来源。为了安全地扩大这些细胞的规模，在动态条件下基于微载体的培养是一种有前途的技术。我们测试了两种微载体的适用性，无孔Cytodex 1和多孔Cytopore 2，培养良好的特征牙髓干细胞（DPSC）使用摇瓶系统。将人DPSC在96孔板中的这些微载体上培养，并在摇瓶中进一步扩增用于放大实验。使用alamarBlue测定法测量细胞活力，同时通过常规和双光子显微镜观察细胞形态。葡萄糖氧化酶/克拉克电极法检测细胞的葡萄糖消耗。DPSC在两个微载体表面上粘附并生长良好，并且也在Cytopore 2的孔中发现。在组织培养板中生长的细胞（静态，非振荡条件）产生7 × 105个细胞/孔。在摇瓶中，静态预孵育促进细胞粘附到微载体。在动态培养条件下（振荡），摇瓶中获得3 × 107个细胞。DPSC耗尽了他们的葡萄糖供应从培养基中的第七天，即使部分分批补料。总之，在动态培养条件下，无孔和多孔微载体都适用于扩大外胚间充质DPSCs。
Ectomesenchymal stem cells derived from the dental pulp are of neural crest origin, and as such are promising sources for cell therapy and tissue engineering. For safe upscaling of these cells, microcarrier-based culturing under dynamic conditions is a promising technology. We tested the suitability of two microcarriers, non-porous Cytodex 1 and porous Cytopore 2, for culturing well characterized dental pulp stem cells (DPSCs) using a shake flask system. Human DPSCs were cultured on these microcarriers in 96-well plates, and further expanded in shake flasks for upscaling experiments. Cell viability was measured using the alamarBlue assay, while cell morphology was observed by conventional and two-photon microscopies. Glucose consumption of cells was detected by the glucose oxidase/Clark-electrode method. DPSCs adhered to and grew well on both microcarrier surfaces and were also found in the pores of the Cytopore 2. Cells grown in tissue culture plates (static, non-shaking conditions) yielded 7 × 105 cells/well. In shake flasks, static preincubation promoted cell adhesion to the microcarriers. Under dynamic culture conditions (shaking) 3 × 107 cells were obtained in shake flasks. The DPSCs exhausted their glucose supply from the medium by day seven even with partial batch-feeding. In conclusion, both non-porous and porous microcarriers are suitable for upscaling ectomesenchymal DPSCs under dynamic culture conditions.
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影响因子：
7.5
作者：
Batsali AK;Pontikoglou C;Koutroulakis D;Pavlaki KI;Damianaki A;Mavroudi I;Alpantaki K;Kouvidi E;Kontakis G;Papadaki HA
通讯作者：
Papadaki HA
影响因子：
5.3
作者：
Földes A;Kádár K;Kerémi B;Zsembery Á;Gyires K;S Zádori Z;Varga G
通讯作者：
Varga G
影响因子：
4.5
作者：
Chen, Allen Kuan-Liang;Chew, Yi Kong;Oh, Steve Kah Weng
通讯作者：
Oh, Steve Kah Weng
影响因子：
3.6
作者：
Kemoun, Philippe;Laurencin-Dalicieux, Sara;Salles, Jean-Pierre
通讯作者：
Salles, Jean-Pierre
影响因子：
2.9
作者：
Caruso, Samia R.;Orellana, Maristela D.;Swiech, Kamilla
通讯作者：
Swiech, Kamilla