Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ.
Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ.
复制标题
DOI：
10.1038/s41467-023-37947-2
复制
发表时间：
2023-05-09
影响因子：
16.6
通讯作者：
Wessel, Gary M.
中科院分区：
文献类型：
--
作者：
Perillo, Margherita;Swartz, S. Zachary;Pieplow, Cosmo;Wessel, Gary M.
器官发生领域的一个基本目标是了解细胞如何组织成管状形状。为此目的，我们建立了海星星星Patiria miniata的水维管器官作为微管发生的模型。在这种动物中，双侧管从发育中的肠道尖端生长出来，并精确地延伸到幼虫的特定部位。这种生长涉及细胞迁移以及不同区域的有丝分裂。细胞增殖需要FGF信号传导，而器官的三维方向依赖于Wnt信号传导。管细胞命运的规范和维持需要Delta/Notch信号传导。此外，我们确定的FGF途径，有助于管形态的靶基因，揭示管生长的分子机制。最后，我们报告说，FGF激活Six 1/2转录因子，这是一个进化古老的分支形态发生的调节器。这项研究揭示了不同的机制tubulogenesis在体内，我们建议，在海星星水血管器官的细胞动力学是一个关键的比较，了解脊椎动物器官的进化。了解细胞如何组织成器官是发育生物学的一项关键工作。在这里，作者介绍了海星星水血管器官作为一个遗传学上易处理的系统，以了解细胞迁移和信号通路在管发生中的贡献。
A fundamental goal in the organogenesis field is to understand how cells organize into tubular shapes. Toward this aim, we have established the hydro-vascular organ in the sea star Patiria miniata as a model for tubulogenesis. In this animal, bilateral tubes grow out from the tip of the developing gut, and precisely extend to specific sites in the larva. This growth involves cell migration coupled with mitosis in distinct zones. Cell proliferation requires FGF signaling, whereas the three-dimensional orientation of the organ depends on Wnt signaling. Specification and maintenance of tube cell fate requires Delta/Notch signaling. Moreover, we identify target genes of the FGF pathway that contribute to tube morphology, revealing molecular mechanisms for tube outgrowth. Finally, we report that FGF activates the Six1/2 transcription factor, which serves as an evolutionarily ancient regulator of branching morphogenesis. This study uncovers distinct mechanisms of tubulogenesis in vivo and we propose that cellular dynamics in the sea star hydro-vascular organ represents a key comparison for understanding the evolution of vertebrate organs. Understanding how cells organize into organs is a key effort in developmental biology. Here the authors introduce the sea star hydrovascular organ as a genetically tractable system to understand the contribution of cell migration and signaling pathways in tubulogenesis.
登录
查看更多内容
细胞迁移的基于丝状的接触刺激驱动组织形态发生。
影响因子：
16.6
作者：
Bischoff MC;Lieb S;Renkawitz-Pohl R;Bogdan S
通讯作者：
Bogdan S
影响因子：
16.6
作者：
Cary GA;McCauley BS;Zueva O;Pattinato J;Longabaugh W;Hinman VF
通讯作者：
Hinman VF
影响因子：
3.7
作者：
Conant, David;Hsiau, Tim;Stoner, Rich
通讯作者：
Stoner, Rich
影响因子：
4.1
作者：
Andrikou C;Iovene E;Rizzo F;Oliveri P;Arnone MI
通讯作者：
Arnone MI
影响因子：
14.9
作者：
Brinkman EK;Chen T;Amendola M;van Steensel B
通讯作者：
van Steensel B