Transcriptional accuracy modeling suggests two-step proofreading by RNA polymerase.
Transcriptional accuracy modeling suggests two-step proofreading by RNA polymerase.
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DOI：
10.1093/nar/gkx849
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发表时间：
2017-11-16
影响因子：
14.9
通讯作者：
Ehrenberg M
中科院分区：
文献类型：
--
作者：
Mellenius H;Ehrenberg M
我们提出了一种新的两步校对机制，即在mRNA转录中进行两轮连续的校对选择。这是基于先前的实验观察，校对RNA聚合酶切割出至少2个核苷酸的转录片段，并且核苷酸误掺入后转录延长异常缓慢。考虑到这些结果，我们扩展了对模板引导核苷酸选择准确性的描述，超越了以前的RNA聚合酶依赖的DNA转录模型。该模型从实验估计的最近邻参数中推导出初始和校对基址选择的准确性。它也被用来估计转录本切割后先前位置的聚合酶重复访问的微小准确性提高。
We suggest a novel two-step proofreading mechanism with two sequential rounds of proofreading selection in mRNA transcription. It is based on the previous experimental observations that the proofreading RNA polymerase cleaves off transcript fragments of at least 2 nt and that transcript elongation after a nucleotide misincorporation is anomalously slow. Taking these results into account, we extend the description of the accuracy of template guided nucleotide selection beyond previous models of RNA polymerase-dependent DNA transcription. The model derives the accuracy of initial and proofreading base selection from experimentally estimated nearest-neighbor parameters. It is also used to estimate the small accuracy enhancement of polymerase revisiting of previous positions following transcript cleavage.
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影响因子：
11.4
作者：
Bochkareva, Aleksandra;Yuzenkova, Yulia;Tadigotla, Vasisht R.;Zenkin, Nikolay
通讯作者：
Zenkin, Nikolay
影响因子：
2.9
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通讯作者：
Aqvist, Johan
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2.9
作者：
BLANK, A;GALLANT, JA;LOEB, LA
通讯作者：
LOEB, LA
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通讯作者：
Kashlev, M
影响因子：
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IZBAN, MG;LUSE, DS
通讯作者：
LUSE, DS