A Novel Regulator of Preadipocyte Differentiation, Transcription Factor TCF21, Functions Partially Through Promoting LPL Expression
A Novel Regulator of Preadipocyte Differentiation, Transcription Factor TCF21, Functions Partially Through Promoting LPL Expression
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前脂肪细胞分化的新型调节因子 TCF21 部分通过促进 LPL 表达发挥作用
DOI：
10.3389/fphys.2019.00458
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发表时间：
2019-04
影响因子：
4
通讯作者：
Hui Li
中科院分区：
文献类型：
--
作者：
Xinyang Zhang;Bohan Cheng;Chang Liu;Zhiqiang Du;Hui Zhang;Ning Wang;Mengqi Wu;Yumao Li;Zhiping Cao;Hui Li
转录因子TCF21此前已被证明在小鼠的白色前脂肪细胞中特异表达。然而，TCF21在脂肪形成过程中的确切生物学功能仍不清楚。在本研究中，我们使用了根据腹脂含量选择的鸡系，观察到瘦肉鸡腹脂中TCF21mRNA和蛋白质水平显著低于肥肉鸡。此外，在体外培养的前脂肪细胞分化过程中，TCF21的表达增加。我们还发现，TCF21基因敲除和过度表达分别抑制和促进了前脂肪细胞的分化，其证据是脂滴积累的适当变化和C/EBPA、LPL和A-FABP表达的改变。染色质免疫沉淀分析和荧光素酶分析表明，TCF21通过直接与LPL启动子中的E-box基序结合来促进LPL的转录。综上所述，这些结果表明TCF21是一种新的前体脂肪细胞分化调节因子，部分是通过直接促进LPL的表达。
The transcription factor TCF21 has been previously shown to be specifically expressed in white preadipocytes in mice. However, the exact biological function of TCF21 in the context of adipogenesis remains unknown. In the current study, we used chicken lines selected based on their abdominal fat content, and observed a significant decrease in TCF21 mRNA and protein levels in the abdominal fat of lean broilers relative to fat broilers. Moreover, TCF21 expression increased throughout preadipocyte differentiation in vitro. We also found that TCF21 knockdown and over-expression attenuated and promoted preadipocyte differentiation, respectively, as evidenced by appropriate changes in lipid droplet accumulation and altered expressions of C/EBPa, LPL, and A-FABP. Additional chromatin immunoprecipitation analyses and luciferase assays demonstrated that TCF21 promotes the transcription of LPL by directly binding to the E-box motif in the LPL promoter. Together, these results show that TCF21 is a novel regulator of preadipocyte differentiation, in part by directly promoting LPL expression.
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影响因子：
2
作者：
R. Dmochowski
通讯作者：
R. Dmochowski
DOI：
10.1016/j.respe.2020.09.004
发表时间：
2020-10-10
期刊：
Revue D'Epidemiologie et De Sante Publique
影响因子：
--
作者：
La rédaction
通讯作者：
La rédaction
影响因子：
9.3
作者：
通讯作者：
--
影响因子：
5.9
作者：
Craighead FL
通讯作者：
Craighead FL