Quantitation of 3-deoxyglucosone levels in human plasma.
Quantitation of 3-deoxyglucosone levels in human plasma.
复制标题
人血浆中 3-脱氧葡萄糖醛酮水平的定量。
DOI：
10.1006/abbi.1997.0117
复制
发表时间：
1997
影响因子：
3.9
通讯作者：
Brown,TR
中科院分区：
文献类型：
--
作者：
Lal,S;Kappler,F;Walker,M;Orchard,TJ;Beisswenger,PJ;Szwergold,BS;Brown,TR
3-脱氧葡萄糖（3DG）是一种活性二羰基，是形成晚期糖基化终产物（AGEs）的重要中间体。AGEs在糖尿病中尤为重要，因为它们与糖尿病并发症的发生有关。因此，3DG的测量可能为这种代谢物在糖尿病并发症病因学中的作用提供有价值的见解。虽然以前已经发表了几种测定人血浆中3DG的方法，但报告的数值存在显著差异（超过30倍）。Knechtet。(拱。物化学。生物物理学报。294,130-137,1992)用GC/MS方法报道了正常血糖的血浆3DG水平为61 nm。与此相反，Niwaet等人（生物化学）。Biophys。Res. comm . 196,837 - 843, 1993)使用完全独立的GC/MS方法报道3DG水平为1800 nmin正常血糖。为了解决这一分歧，并满足对这种二羰基的可靠测定的需要，适合于绝对定量，设计了一种GC/MS程序来测量它。血浆样品通过超滤或Niwaet al.（Biochem）描述的添加乙醇来脱蛋白。Biophys。Res. communi .196, 837-843, 1993)。超滤液或上清液中的3DG与2,3-二氨基萘偶联生成稳定的加合物，然后将其转化为硅醚，并进行GC/MS分析。分析物采用选择的离子监测仪在anm/zof 295和306下进行监测，并用[U-13C]3DG内标进行定量。使用该方法，超滤脱蛋白血浆中3DG水平从正常血糖患者的58.5±14 (SD) nmin显著升高到I型糖尿病患者的98.5±34 (SD) nmin。当使用乙醇对血浆进行脱蛋白处理时，正常血糖血浆中的3DG水平与Niwaet等人报道的水平相似（1710±750 (SD) nm）。这些结果表明，通过超滤测量的3DG水平可能代表自由循环的3DG，而通过乙醇提取获得的3DG水平可能代表与大分子（假设是蛋白质）结合的3DG的一种形式。
3-Deoxyglucosone (3DG), a reactive dicarbonyl, is an important intermediate in the formation of advanced glycation end products (AGEs). The AGEs are particularly important in diabetes since they have been correlated with the development of diabetic complications. Consequently, measurements of 3DG are likely to provide valuable insights into the role of this metabolite in the etiology of diabetic complications. While several methods of 3DG quantitation in human plasma have been previously published, a significant discrepancy (over 30-fold) exists in the reported values. Knechtet al.(Arch. Biochem. Biophys.294, 130–137, 1992) have reported the levels of plasma 3DG in normoglycemics to be 61 nm, using a GC/MS procedure. In contrast to this, Niwaet al.(Biochem. Biophys. Res. Commun.196, 837–843, 1993) reported 3DG levels to be 1800 nmin normoglycemics, using a totally independent GC/MS method. To resolve this disagreement and fill the need for a robust assay for this dicarbonyl, suitable for absolute quantitation, a GC/MS procedure was devised for its measurement. Plasma samples were deproteinized either by ultrafiltration or by addition of ethanol as described by Niwaet al.(Biochem. Biophys. Res. Commun.196, 837–843, 1993). 3DG in the ultrafiltrate or the supernatant was conjugated with 2,3-diaminonaphthalene to produce a stable adduct which was then converted to a silyl ether and analyzed by GC/MS. The analyte was monitored by selected ion monitoring at anm/zof 295 and 306 and quantitated using an internal standard of [U-13C]3DG. Using this approach, 3DG levels in plasma deproteinized by ultrafiltration were found to be significantly elevated from 58.5 ± 14 (SD) nmin normoglycemics to 98.5 ± 34 (SD) nmin type I diabetics. When deproteinization of the plasma was carried out using ethanol, the levels of 3DG from normoglycemic plasma were similar to those reported by Niwaet al.(1710 ± 750 (SD) nm). These results suggest that 3DG levels measured by ultrafiltration may represent the free circulating 3DG and those obtained by ethanol extraction may represent aform of 3DG bound to a macromolecule (presumbaly protein).
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DOI：
10.1271/bbb1961.52.1451
发表时间：
1988
期刊：
Agricultural and biological chemistry
影响因子：
--
作者：
Dong;F. Hayase;H. Kato
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DOI：
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发表时间：
1969
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DOI：
--
发表时间：
1994
期刊：
影响因子：
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作者：
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影响因子：
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作者：
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DOI：
--
发表时间：
1987
期刊：
影响因子：
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作者：
H. Kato;Dong;F. Hayase
通讯作者：
F. Hayase