A global view of protein expression in human cells, tissues, and organs.
A global view of protein expression in human cells, tissues, and organs.
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DOI：
10.1038/msb.2009.93
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发表时间：
2009
影响因子：
9.9
通讯作者：
Uhlen, Mathias
中科院分区：
文献类型：
--
作者：
Ponten, Fredrik;Gry, Marcus;Fagerberg, Linn;Lundberg, Emma;Asplund, Anna;Berglund, Lisa;Oksvold, Per;Bjorling, Erik;Hober, Sophia;Kampf, Caroline;Navani, Sanjay;Nilsson, Peter;Ottosson, Jenny;Persson, Anja;Wernerus, Henrik;Wester, Kenneth;Uhlen, Mathias
关键词：
定义组织和器官的蛋白质谱对于理解人体中各种细胞类型的独特特征至关重要。在这项研究中，我们报告了48种人体组织和45种人体细胞系中4842种蛋白质谱的解剖学综合分析。对超过200万张手动注释的高分辨率基于免疫化学的图像进行的详细分析显示，在大多数细胞和组织中表达的蛋白质比例很高（>65%），在任何单一细胞类型中检测到的蛋白质很少（<2%）。同样，共聚焦显微镜在三种人类细胞系中检测到超过70%的分析蛋白质的表达。尽管这种无处不在的表达，层次聚类分析，基于全球蛋白质表达模式，显示分析的细胞仍然可以细分成组，根据目前的组织学和细胞分化的概念。这项研究表明，组织特异性是通过在空间和时间上精确调节蛋白质水平来实现的，体内不同组织获得其独特的特征不是通过控制表达哪些蛋白质，而是通过控制每种蛋白质的产生量。
Defining the protein profiles of tissues and organs is critical to understanding the unique characteristics of the various cell types in the human body. In this study, we report on an anatomically comprehensive analysis of 4842 protein profiles in 48 human tissues and 45 human cell lines. A detailed analysis of over 2 million manually annotated, high-resolution, immunohistochemistry-based images showed a high fraction (>65%) of expressed proteins in most cells and tissues, with very few proteins (<2%) detected in any single cell type. Similarly, confocal microscopy in three human cell lines detected expression of more than 70% of the analyzed proteins. Despite this ubiquitous expression, hierarchical clustering analysis, based on global protein expression patterns, shows that the analyzed cells can be still subdivided into groups according to the current concepts of histology and cellular differentiation. This study suggests that tissue specificity is achieved by precise regulation of protein levels in space and time, and that different tissues in the body acquire their unique characteristics by controlling not which proteins are expressed but how much of each is produced.
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