Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation.
Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation.
复制标题
成年干细胞多能与DNA甲基化独立于成年干细胞多能性是必需的。
DOI：
10.1016/j.ydbio.2013.09.020
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发表时间：
2013-12-01
影响因子：
2.7
通讯作者：
Aboobaker, A. Aziz
中科院分区：
文献类型：
--
作者：
Jaber-Hijazi, Farah;Lo, Priscilla J. K. P.;Mihaylova, Yuliana;Foster, Jeremy M.;Benner, Jack S.;Romero, Belen Tejada;Chen, Chen;Malla, Sunir;Solana, Jordi;Ruzov, Alexey;Aboobaker, A. Aziz
涡虫成体干细胞（pASCs）或新母细胞是研究干细胞进化和多能性的理想系统，因为它们具有无与伦比的再生能力。我们希望了解pASCs中分化和多能性的控制，并了解这些机制在两侧体中的保守性、趋同性或差异性。本研究表明，涡虫甲基cpg结合结构域2/3 （mbd2/3）基因是pASC在再生和组织稳态过程中分化所必需的。基因组中没有可检测到的5-甲基胞嘧啶（5mC）水平，我们没有发现潜在的DNA甲基化酶的作用。我们得出结论，MBD蛋白可能在广泛控制动物干细胞多能性方面具有古老的作用，但DNA甲基化与涡虫干细胞分化无关。地中海拟涡虫中存在一个单一的祖先MBD2/3蛋白。地中海S.基因组没有普遍的胞嘧啶甲基化。MBD2/3是多能干细胞向多个但不是所有细胞系分化所必需的。MBD2/3可能在调节干细胞多能性方面具有祖先作用。
Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5mC) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation. A single ancestral MBD2/3 protein is present in the planarian Schmidtea mediterranea. The genome of S. mediterranea does not have pervasive cytosine methylation. MBD2/3 is required for pluripotent stem cell differentiation down multiple but not all cell lineages. MBD2/3 may have had an ancestral role in regulating stem cell pluripotency.
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影响因子：
2.4
作者：
Cardona, A;Fernández, J;Romero, R
通讯作者：
Romero, R
影响因子：
4.4
作者：
Geyer KK;Chalmers IW;Mackintosh N;Hirst JE;Geoghegan R;Badets M;Brophy PM;Brehm K;Hoffmann KF
通讯作者：
Hoffmann KF
影响因子：
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作者：
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通讯作者：
Ruzov, Alexey
影响因子：
0.7
作者：
Gonzalez-Estevez, Cristina;Arseni, Varvara;Aboobaker, A. Aziz
通讯作者：
Aboobaker, A. Aziz
影响因子：
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作者：
Guo, Tingxia;Peters, Antoine H. F. M.;Newmark, Phillip A.
通讯作者：
Newmark, Phillip A.