Ex Vivo Imaging of Ultrasound-Stimulated Metabolic Activity in Rat Pancreatic Slices.
Ex Vivo Imaging of Ultrasound-Stimulated Metabolic Activity in Rat Pancreatic Slices.
复制标题
大鼠胰腺切片中超声刺激的代谢活性的离体成像。
DOI：
10.1016/j.ultrasmedbio.2020.10.021
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发表时间：
2021-03
影响因子：
2.9
通讯作者：
Zderic V
中科院分区：
文献类型：
--
作者：
Chen AW;Jeremic A;Zderic V
先前已显示超声波在培养的大鼠β细胞中产生可逆刺激作用。在这里，我们量化和评估动态代谢变化，在原位胰腺切片模型诱发的超声应用。接种后，使用共聚焦显微镜在488 nm和633 nm下对胰腺切片成像，以分别对脂胺脱氢酶（Lip-DH）自体荧光和远红荧光成像。在800 kHz和1 MHz下以0.5和1 W/cm 2的强度施加超声。另外，在脉冲方案中以lW/cm 2施加800 kHz。也不进行超声（对照）和葡萄糖应用实验。在处理应用之前和之后的荧光信号的差异是用于分析的度量。使用远红荧光的实验组比较显示，在胰岛中，所有实验组与对照组之间存在显著差异（p<0.05），在胰腺外分泌组织中，所有超声实验组与对照组之间存在显著差异（p<0.05）。然而，这种差异之间的反应控制和葡萄糖并不存在于外分泌组织。我们还使用Lip-DH自体荧光观察到，与外分泌组织相比，葡萄糖在胰岛组织中产生显著增加的代谢反应（p<0.05）。与连续超声应用相比，脉冲超声似乎以更一致的方式增加胰腺切片中的代谢活性。我们的结果表明，治疗性超声可能对胰岛具有类似于葡萄糖的刺激代谢作用。
Ultrasound has been shown previously to produce a reversible stimulatory effect in cultured rat β-cells. Here, we quantified and assessed dynamical metabolic changes in an in situ pancreatic slice model evoked by ultrasound application. After plating, pancreas slices were imaged using a confocal microscope at 488 nm and 633 nm to image lipodamine dehydrogenase (Lip-DH) autofluorescence and a far red fluorescence respectively. Ultrasound was applied at intensities of 0.5 and 1 W/cm2 at both 800 kHz and 1 MHz. Additionally 800 kHz at 1 W/cm2 was applied in a pulsing scheme. No ultrasound (control) and glucose application experiments were performed as well. A difference in fluorescence signal before and after treatment application was the metric for analysis. Comparison of experimental groups using far red fluorescence revealed significant differences between all experimental groups and control in the islet (p<0.05) and between all ultrasound experimental groups and control (p<0.05) in pancreatic exocrine tissue. However, this difference in response between control and glucose did not exist in the exocrine tissue. We also observed using Lip-DH autofluorescence that glucose produces a significantly increased metabolic response in islet tissue compared to exocrine tissue (p<0.05). Pulsed ultrasound appeared to increase metabolic activity in the pancreatic slice in a more consistent manner compared to continuous ultrasound application. Our results indicate that therapeutic ultrasound may have a stimulatory metabolic effect on the pancreatic islets similar to glucose.
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影响因子：
2.9
作者：
DALECKI, D;KELLER, BB;CARSTENSEN, EL
通讯作者：
CARSTENSEN, EL
影响因子：
2.9
作者：
Hensel, Karin;Mienkina, Martin P.;Schmitz, Georg
通讯作者：
Schmitz, Georg
DOI：
10.1186/s40349-017-0108-9
发表时间：
2017-12-05
期刊：
JOURNAL OF THERAPEUTIC ULTRASOUND
影响因子：
--
作者：
Castellanos, Ivan Suarez;Singh, Tania;Zderic, Vesna
通讯作者：
Zderic, Vesna
影响因子：
2.9
作者：
DELIUS, M;HOFFMANN, E;CONZEN, P
通讯作者：
CONZEN, P
影响因子：
3.2
作者：
Baschong, W;Suetterlin, R;Laeng, RH
通讯作者：
Laeng, RH