Noninvasively Imaging pH at the Surface of Implanted Orthopedic Devices with X-ray Excited Luminescence Chemical Imaging.
Noninvasively Imaging pH at the Surface of Implanted Orthopedic Devices with X-ray Excited Luminescence Chemical Imaging.
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DOI：
10.1021/acssensors.9b00962
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发表时间：
2019-09-27
期刊：
影响因子：
8.9
通讯作者：
Anker, Jeffrey N.
中科院分区：
文献类型：
--
作者：
Uzair, Unaiza;Benza, Donald;Behrend, Caleb J.;Anker, Jeffrey N.
Implanted medical device-associated infections are a leading cause of fixation failure and early diagnosis is key to successful treatment. During infection, acidosis near the implant plays a role in antibiotic resistance and low pH is a potential infection indicator. Herein, we describe a pH sensor which attaches to implants to noninvasively image local pH with high spatial resolution. The sensor has two layers: a scintillator layer which emits 620 and 700 nm light upon X-ray irradiation, and a pH indicator layer containing bromocresol green dye that absorbs 620 nm luminescence in neutral/basic pH and passes 700 nm light at all pH. We also developed a dedicated imaging system capable of scanning relatively large specimens through thick tissue. A focused X-ray beam irradiates one spot on the sensor, and the 620 to 700 nm peak ratio is measured to determine local pH; images are acquired by scanning the X-ray beam across the surface and measuring pH point-by-point. The sensor was covered with varying thickness slices of chicken breast tissue (0-19 mm) to evaluate how tissue affects peak intensity and ratio. Thick tissue attenuated both 620 nm and 700 nm light, with more attenuation at 620 nm than 700 nm. Although this spectral distortion shifted the pH calibration curve, the effect could be corrected for using a scintillator film region with no pH-indicator layer as a spectral reference. The sensor was attached to an orthopedic plate affixed to a human cadaveric tibia and imaged through tissue. The approach provides both high spatial resolution from focused X-ray excitation, and surface chemical specificity from the indicator dye providing a tool for imaging local pH through tissue. Authors are required to submit a graphic entry for the Table of Contents (TOC) that, in conjunction with the manuscript title, should give the reader a representative idea of one of the following: A key structure, reaction, equation, concept, or theorem, etc., that is discussed in the manuscript. Consult the journal’s Instructions for Authors for TOC graphic specifications.
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DOI：
10.1039/c2cp41858d
发表时间：
2012-10-21
期刊：
Physical chemistry chemical physics : PCCP
影响因子：
--
作者：
Chen H;Rogalski MM;Anker JN
通讯作者：
Anker JN
影响因子：
3.1
作者：
Broekhuizen, C. A. N.;Sta, M.;Zaat, S. A. J.
通讯作者：
Zaat, S. A. J.
影响因子：
6.2
作者：
Konttinen, YT;Takagi, M;Santavirta, S
通讯作者：
Santavirta, S
影响因子：
7.4
作者：
Dansby-Sparks, Royce N.;Jin, Jun;Xue, Zi-Ling
通讯作者：
Xue, Zi-Ling
DOI：
10.3109/17453677608998968
发表时间：
1976-01-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
RAAHAVE, D
通讯作者：
RAAHAVE, D