Peptide hydrogelation and cell encapsulation for 3D culture of MCF-7 breast cancer cells.
Peptide hydrogelation and cell encapsulation for 3D culture of MCF-7 breast cancer cells.
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DOI：
10.1371/journal.pone.0059482
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nguyen TA
中科院分区：
文献类型：
--
作者：
Huang H;Ding Y;Sun XS;Nguyen TA
三维（3D）细胞培养在肿瘤生物学中起着不可估量的作用，其通过提供类似于体内的微环境和对治疗剂的反应。在许多已建立的3D支架中，水凝胶表现出作为3D细胞培养基质的独特性质。大多数现有的预凝胶溶液在生理条件下受到限制，例如不期望的pH或温度。在这里，我们报告了一种肽水凝胶，其显示出作为3D细胞培养的体外基质的上级生理特性。3D基质可以通过将自组装肽直接与细胞培养基混合而无需任何pH或温度调节来实现。动态流变学研究的结果表明，这种水凝胶可以通过移液多次递送，而不会永久破坏水凝胶结构，表明水凝胶的可变形性和重塑能力。人上皮癌细胞MCF-7在水凝胶化期间均匀地包封在水凝胶基质中。与二维（2D）单层培养相比，驻留在水凝胶基质中的细胞在3D形成中生长为肿瘤样簇。使用3D水凝胶中的MCF-7细胞分析与细胞形态、存活、增殖和凋亡相关的相关参数。有趣的是，顺铂（一种抗癌药物）的处理可以导致水凝胶中MCF-7簇的细胞活力显著降低。对顺铂的反应是剂量和时间依赖性的，表明水凝胶用于药物测试的潜在用途。共聚焦显微镜和蛋白质印迹的结果表明，从水凝胶分离的细胞是适合下游蛋白质组学分析。结果提供了证据表明，这种肽水凝胶是一种有前途的药物测试的3D细胞培养材料。
Three-dimensional (3D) cell culture plays an invaluable role in tumor biology by providing in vivo like microenviroment and responses to therapeutic agents. Among many established 3D scaffolds, hydrogels demonstrate a distinct property as matrics for 3D cell culture. Most of the existing pre-gel solutions are limited under physiological conditions such as undesirable pH or temperature. Here, we report a peptide hydrogel that shows superior physiological properties as an in vitro matrix for 3D cell culture. The 3D matrix can be accomplished by mixing a self-assembling peptide directly with a cell culture medium without any pH or temperature adjustment. Results of dynamic rheological studies showed that this hydrogel can be delivered multiple times via pipetting without permanently destroying the hydrogel architecture, indicating the deformability and remodeling ability of the hydrogel. Human epithelial cancer cells, MCF-7, are encapsulated homogeneously in the hydrogel matrix during hydrogelation. Compared with two-dimensional (2D) monolayer culture, cells residing in the hydrogel matrix grow as tumor-like clusters in 3D formation. Relevant parameters related to cell morphology, survival, proliferation, and apoptosis were analyzed using MCF-7 cells in 3D hydrogels. Interestingly, treatment of cisplatin, an anti-cancer drug, can cause a significant decrease of cell viability of MCF-7 clusters in hydrogels. The responses to cisplatin were dose- and time-dependent, indicating the potential usage of hydrogels for drug testing. Results of confocal microscopy and Western blotting showed that cells isolated from hydrogels are suitable for downstream proteomic analysis. The results provided evidence that this peptide hydrogel is a promising 3D cell culture material for drug testing.
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影响因子：
14
作者：
Branco, Monica C.;Pochan, Darrin J.;Wagner, Norman J.;Schneider, Joel P.
通讯作者：
Schneider, Joel P.
DOI：
10.1073/pnas.1735463100
发表时间：
2003-10-28
影响因子：
11.1
作者：
Levenberg, S;Huang, NF;Langer, R
通讯作者：
Langer, R
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
6.2
作者：
Chawla, Kanika;Yu, Ting-Bin;Liao, Sophia W.;Guan, Zhibin
通讯作者：
Guan, Zhibin
影响因子：
19
作者：
Rapaport, Hanna;Grisaru, Haviv;Silberstein, Tova
通讯作者：
Silberstein, Tova