The Halotolerant Rhizobacterium-Pseudomonas koreensis MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (Glycine max L.).
The Halotolerant Rhizobacterium-Pseudomonas koreensis MU2 Enhances Inorganic Silicon and Phosphorus Use Efficiency and Augments Salt Stress Tolerance in Soybean (Glycine max L.).
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DOI：
10.3390/microorganisms8091256
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发表时间：
2020-08-19
期刊：
影响因子：
4.5
通讯作者：
Lee IJ
中科院分区：
文献类型：
--
作者：
Adhikari A;Khan MA;Lee KE;Kang SM;Dhungana SK;Bhusal N;Lee IJ
优化植物的养分利用对于在生物和非生物胁迫下的可持续产量至关重要。由于硅和磷被认为是植物生长的关键元素，本研究通过接种根际菌株韩国假单胞菌MU2，评估了盐胁迫下大豆植株有效的硅和磷补充策略。MU2的筛选分析显示其高耐盐潜力，其溶解硅酸盐和磷酸盐。分离物MU2在正常和盐胁迫条件下在纯培养中产生赤霉酸（GA1，GA3）和有机酸（苹果酸，柠檬酸，乙酸和酒石酸）。MU 2、硅和磷的联合施用显着提高了盐胁迫条件下大豆植株地上部对硅和磷的吸收，减少了70%的Na+离子流入，并使K+吸收增加了46%。接种MU2可上调大豆抗盐基因GmST 1、GmSALT 3和GmAKT2的表达，显著降低内源激素脱落酸和茉莉酸的含量，提高水杨酸的含量。此外，接种MU2还通过减少脂质过氧化和脯氨酸来增强宿主的抗氧化系统，同时提高还原型谷胱甘肽的含量。此外，接种MU2促进了大豆植株的根和茎长度、植株生物量和叶绿素含量。这些结果表明，MU2可能是一种潜在的生物肥料催化剂，用于放大硅和磷肥的利用效率，以缓解盐胁迫。
Optimizing nutrient usage in plants is vital for a sustainable yield under biotic and abiotic stresses. Since silicon and phosphorus are considered key elements for plant growth, this study assessed the efficient supplementation strategy of silicon and phosphorus in soybean plants under salt stress through inoculation using the rhizospheric strain—Pseudomonas koreensis MU2. The screening analysis of MU2 showed its high salt-tolerant potential, which solubilizes both silicate and phosphate. The isolate, MU2 produced gibberellic acid (GA1, GA3) and organic acids (malic acid, citric acid, acetic acid, and tartaric acid) in pure culture under both normal and salt-stressed conditions. The combined application of MU2, silicon, and phosphorus significantly improved silicon and phosphorus uptake, reduced Na+ ion influx by 70%, and enhanced K+ uptake by 46% in the shoots of soybean plants grown under salt-stress conditions. MU2 inoculation upregulated the salt-resistant genes GmST1, GmSALT3, and GmAKT2, which significantly reduced the endogenous hormones abscisic acid and jasmonic acid while, it enhanced the salicylic acid content of soybean. In addition, MU2 inoculation strengthened the host’s antioxidant system through the reduction of lipid peroxidation and proline while, it enhanced the reduced glutathione content. Moreover, MU2 inoculation promoted root and shoot length, plant biomass, and the chlorophyll content of soybean plants. These findings suggest that MU2 could be a potential biofertilizer catalyst for the amplification of the use efficiency of silicon and phosphorus fertilizers to mitigate salt stress.
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影响因子：
4.6
作者：
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通讯作者：
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影响因子：
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通讯作者：
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作者：
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通讯作者：
Lee IJ