Plant growth promoting rhizobacteria Dietzia natronolimnaea modulates the expression of stress responsive genes providing protection of wheat from salinity stress.
Plant growth promoting rhizobacteria Dietzia natronolimnaea modulates the expression of stress responsive genes providing protection of wheat from salinity stress.
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DOI：
10.1038/srep34768
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发表时间：
2016-10-06
影响因子：
4.6
通讯作者：
Kalra A
中科院分区：
文献类型：
--
作者：
Bharti N;Pandey SS;Barnawal D;Patel VK;Kalra A
促进植物根际生长的细菌（PGPR）为可持续农业带来了光明的前景。在这里，我们展示了一种产生类胡萝卜素的耐盐 PGPR Dietzia natronolimnaea STR1，它通过调节负责植物耐盐性的转录机制来保护小麦植物免受盐胁迫。表达研究证实了 ABA 信号级联的参与，因为 TaABARE 和 TaOPR1 在接种 PGPR 的植物中上调，导致 TaMYB 和 TaWRKY 表达诱导，随后刺激大量胁迫相关基因的表达。与未接种的对照植物相比，在接种 PGPR 的植物中观察到与促进耐盐性相关的 TaST（一种盐胁迫诱导基因）的表达增强。 SOS 途径相关基因（SOS1 和 SOS4）的表达在施用 PGPR 的小麦芽和根系统中受到调节。在接种 PGPR 的植物中观察到离子转运蛋白 TaNHX1、TaHAK 和 TaHKT1 的组织特异性反应。接种 PGPR 的小麦植株中各种抗氧化酶（如 APX、MnSOD、CAT、POD、GPX 和 GR）基因表达的增强以及脯氨酸含量的提高有助于提高对盐胁迫的耐受性。总体而言，这些结果表明耐盐 PGPR 介导的盐度耐受性是一种复杂的现象，涉及 ABA 信号、SOS 途径、离子转运蛋白和抗氧化机制的调节。
Plant growth promoting rhizobacteria (PGPR) hold promising future for sustainable agriculture. Here, we demonstrate a carotenoid producing halotolerant PGPR Dietzia natronolimnaea STR1 protecting wheat plants from salt stress by modulating the transcriptional machinery responsible for salinity tolerance in plants. The expression studies confirmed the involvement of ABA-signalling cascade, as TaABARE and TaOPR1 were upregulated in PGPR inoculated plants leading to induction of TaMYB and TaWRKY expression followed by stimulation of expression of a plethora of stress related genes. Enhanced expression of TaST, a salt stress-induced gene, associated with promoting salinity tolerance was observed in PGPR inoculated plants in comparison to uninoculated control plants. Expression of SOS pathway related genes (SOS1 and SOS4) was modulated in PGPR-applied wheat shoots and root systems. Tissue-specific responses of ion transporters TaNHX1, TaHAK, and TaHKT1, were observed in PGPR-inoculated plants. The enhanced gene expression of various antioxidant enzymes such as APX, MnSOD, CAT, POD, GPX and GR and higher proline content in PGPR-inoculated wheat plants contributed to increased tolerance to salinity stress. Overall, these results indicate that halotolerant PGPR-mediated salinity tolerance is a complex phenomenon that involves modulation of ABA-signalling, SOS pathway, ion transporters and antioxidant machinery.
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影响因子：
9.4
作者：
Baltruschat, Helmut;Fodor, Jozsef;Skoczowski, Andrzej
通讯作者：
Skoczowski, Andrzej
小麦氧化植物二烯酸还原酶基因 TaOPR1 通过增强脱落酸信号传导和活性氧清除赋予耐盐性
影响因子：
7.4
作者：
Dong, Wei;Wang, Mengcheng;Xia, Guangmin
通讯作者：
Xia, Guangmin
影响因子：
6.5
作者：
Barnawal, Deepti;Bharti, Nidhi;Kalra, Alok
通讯作者：
Kalra, Alok
影响因子：
4.8
作者：
Bharti, Nidhi;Barnawal, Deepti;Kalra, Alok
通讯作者：
Kalra, Alok
影响因子：
2.8
作者：
Agarwal, Parinita;Reddy, M. P.;Chikara, Jitendra
通讯作者：
Chikara, Jitendra