Differentiation stimuli induce receptors for plasma fibronectin on the human myelomonocytic cell line HL-60.
Differentiation stimuli induce receptors for plasma fibronectin on the human myelomonocytic cell line HL-60.
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分化刺激诱导人骨髓单核细胞系 HL-60 上血浆纤连蛋白受体。
DOI：
10.1182/blood.v64.4.858.858
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发表时间：
1984
期刊：
影响因子：
20.3
通讯作者：
E. Brown
中科院分区：
文献类型：
--
作者：
C. Pommier;J. O’Shea;T. Chused;T. Takahashi;M. Ochoa;T. Nutman;C. Bianco;E. Brown
血浆纤维连接蛋白（Fn）诱导人外周血单核细胞对C3b调理的绵羊红细胞（EC3b）的吞噬作用。然而，Fn不会诱导人多形核白细胞（PMN）对EC3b的红细胞吞噬作用，除非PMN已暴露于C5a或N -甲酰 - 甲硫氨酰 - 亮氨酰 - 苯丙氨酸。由于这种差异，研究Fn与具有分化为粒细胞或单核细胞能力的细胞的结合是非常有趣的。因此，我们研究了在HL - 60细胞沿单核细胞样或粒细胞样途径体外分化前后，Fn与人类髓单核细胞系HL - 60结合的结果。在未分化的HL - 60细胞上未发现Fn受体，但几种分化诱导剂促进了Fn包被的微球（Fn - ms）与HL - 60的结合。在用二甲基亚砜（DMSO）诱导分化后4到5天以及用佛波醇酯（PMA）诱导后2天，Fn - ms结合达到峰值。此外，当沿单核细胞样或粒细胞样途径分化的细胞暴露于Fn时，它们对IgG包被的红细胞（EA）和EC3b的吞噬作用显著增加。比较抗Fn单克隆抗体对Fn - ms与单核细胞、PMN和HL - 60结合的影响表明，相同的单克隆抗体可阻断所有三种细胞类型的Fn - ms结合和Fn诱导的EC3b吞噬作用。两种针对PMN和单核细胞膜抗原的单克隆抗体M1/70和A6F10抑制Fn - ms结合。它们也阻断这些细胞对Fn诱导的EC3b摄取。然而，这两种抗体都不阻断分化的HL - 60细胞的Fn - ms结合或EC3b摄取。我们得出结论，分化的HL - 60细胞表达功能活跃的Fn受体，类似于单核细胞和活化的PMN，但在与M1/70和A6F10识别的抗原的关联方面与正常细胞不同。
Plasma fibronectin (Fn) induces phagocytosis of C3b-opsonized sheep erythrocytes (EC3b) by human peripheral blood monocytes. However, Fn does not induce erythrophagocytosis of EC3b by human polymorphonuclear leukocytes (PMN), unless the PMN have been exposed to C5a or N-formyl-methionyl-leucyl-phenylalanine. Because of this difference, it is of great interest to examine Fn binding to cells that possess the capacity to differentiate into either granulocytes or monocytes. Hence, we have examined the consequences of Fn binding to the human myelomonocytic cell line, HL-60, both before and after in vitro differentiation of the HL-60, along a monocytoid or a granulocytoid pathway. Fn receptors were not found on undifferentiated HL-60, but several differentiating agents promoted the HL-60 binding of Fn-coated microspheres (Fn-ms). The peak of Fn-ms binding occurred four to five days after the induction of differentiation with dimethylsulfoxide (DMSO), and two days after induction by PMA. In addition, cells that differentiated along either the monocytoid or the granulocytoid pathway showed a marked increase in the phagocytosis of both IgG-coated erythrocytes (EA) and EC3b when they were exposed to Fn. Comparison of the effects of anti-Fn monoclonals on the binding of Fn-ms to the monocytes, PMN, and HL-60 showed that the same monoclonals block Fn-ms-binding and Fn-induced EC3b phagocytosis by all three cell types. Two monoclonal antibodies, M1/70 and A6F10, directed against membrane antigens on PMN and monocytes, inhibited Fn-ms binding. Both also blocked Fn-induced EC3b ingestion by these cells. However, neither antibody blocked Fn-ms binding or EC3b ingestion by differentiated HL-60. We conclude that differentiated HL-60 cells express functionally active Fn receptors, similar to monocytes and activated PMN, which, nonetheless, differ from normal cells in their association with the antigens recognized by M1/70 and A6F10.
影响因子：
20.3
作者：
D. Ferrero;S. Pessano;G. Pagliardi;G. Rovera
通讯作者：
D. Ferrero;S. Pessano;G. Pagliardi;G. Rovera
HL-60 细胞系分化过程中人骨髓特异性抗原的表达和调节。
影响因子：
20.3
作者：
Graziano,RF;Ball,ED;Fanger,MW
通讯作者：
Fanger,MW
纤连蛋白介导金黄色葡萄球菌与人中性粒细胞的附着。
影响因子：
20.3
作者：
Proctor,RA;Prendergast,E;Mosher,DF
通讯作者：
Mosher,DF