Minimally invasive dynamic imaging of ions and metabolites in living cells.
Minimally invasive dynamic imaging of ions and metabolites in living cells.
复制标题
活细胞中离子和代谢物的微创动态成像。
DOI：
10.1016/j.pbi.2004.03.015
复制
发表时间：
2004
影响因子：
9.5
通讯作者：
W. Frommer
中科院分区：
文献类型：
--
作者：
M. Fehr;D. Ehrhardt;S. Lalonde;W. Frommer
到2010年，预计生物化学功能将被分配给大约30 000个拟南芥基因的许多产物。此外，突变体的系统分析将提供对基因产物的生物学功能的洞察。代谢组学技术通过测试细胞离子和代谢物模式的变化来补充这些方法，为构建细胞和全植物代谢模型提供重要信息。然而，一组重要的信息，特别是相关的多细胞生物仍然缺乏，即，知识的细胞和亚细胞的变化代谢物水平。最近开发的基于蛋白质的代谢物纳米传感器将有助于缩小这一差距，提供了一套工具，可用于确定细胞溶质和亚细胞代谢物水平在真实的时间使用荧光显微镜。未来的一个主要挑战是应用这些纳米传感器来量化植物细胞和组织中的代谢物水平。
By 2010, it is expected that biochemical functions will be assigned to many of the products of the approximately 30 000 Arabidopsis genes. Moreover, systematic analysis of mutants will provide insight into the biological function of the gene products. Metabolomic technologies complement these approaches by testing for changes in cellular ion and metabolite patterns, providing essential information for the construction of cellular and whole-plant models of metabolism. However, one important set of information that is especially relevant for multicellular organisms is still lacking, that is, knowledge of the cellular and subcellular variation in metabolite levels. The recent development of protein-based nanosensors for metabolites will help to close this gap by providing a set of tools that can be used to determine cytosolic and subcellular metabolite levels in real time using fluorescence-based microscopy. A major challenge for the future is the application of these nanosensors to quantify metabolite levels in plant cells and tissues.
影响因子：
15
作者：
Shafer-Peltier, KE;Haynes, CL;Van Duyne, RP
通讯作者：
Van Duyne, RP