Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells.
Efficient c-kit receptor-targeted gene transfer to primary human CD34-selected hematopoietic stem cells.
复制标题
将 c-kit 受体靶向基因有效转移至原代人类 CD34 选择的造血干细胞。
DOI：
10.1128/jvi.75.21.10393-10400.2001
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发表时间：
2001
影响因子：
5.4
通讯作者：
Schwarzenberger,P
中科院分区：
文献类型：
--
作者：
Zhong,Q;Oliver,P;Huang,W;Good,D;LaRussa,V;Zhang,Z;Cork,JR;Veith,RW;Theodossiou,C;Kolls,JK;Schwarzenberger,P
We have previously reported effective gene transfer with a targeted molecular conjugate adenovirus vector through the c-kit receptor in hematopoietic progenitor cell lines. However, a c-kit-targeted recombinant retroviral vector failed to transduce cells, indicating the existence of significant differences for c-kit target gene transfer between these two viruses. Here we demonstrate that conjugation of an adenovirus to a c-kit-retargeted retrovirus vector enables retroviral transduction. This finding suggests the requirement of endosomalysis for successful c-kit-targeted gene transfer. Furthermore, we show efficient gene transfer to, and high transgene expression (66%) in, CD34-selected, c-kit+human peripheral blood stem cells using a c-kit-targeted adenovirus vector. These findings may have important implications for future vector development in c-kit-targeted stem cell gene transfer.
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作者：
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