Localization of epididymal secretory proteins on rat spermatozoa.
Localization of epididymal secretory proteins on rat spermatozoa.
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附睾分泌蛋白在大鼠精子上的定位。
DOI：
10.1530/jrf.0.0690651
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
K. Tiver
中科院分区：
文献类型：
--
作者：
D. Brooks;K. Tiver
取大鼠睾丸和附睾尾精子表面用放射性碘标记。用抗血清免疫沉淀放射性碘化精子的洗涤剂提取物，对附睾特异性蛋白进行免疫沉淀，然后用聚丙烯酰胺凝胶电泳，发现在附睾转运过程中与精子相关的两个蛋白（Mr为27 000和28 000的D和E）。免疫荧光显微镜观察到这些蛋白位于头部表面的有限区域。在半乳糖氧化酶存在的情况下，通过与氢化[3H]钠反应，在附睾尾精子上标记了具有相似分子量的蛋白质，而不是来自睾丸的蛋白质。然而，蛋白质D和E的抗体免疫沉淀失败，以及在二维凝胶电泳上的不一致迁移，确定了这些蛋白质的非同一性。与蛋白D和E相比，另外两种主要的附睾分泌蛋白（Mr . 16000的蛋白B和C）在附睾运输过程中与精子的关联程度相对较小。
Spermatozoa from the testis and cauda epididymidis of the rat were surface labelled with radioactive iodide. Detergent extracts of radioiodinated spermatozoa immunoprecipitated with antisera against specific epididymal proteins, followed by polyacrylamide gel electrophoresis, revealed two proteins (D and E of Mr 27 000 and 28 000, respectively) which became associated with spermatozoa during epididymal transit. These proteins were observed by immunofluorescence microscopy to be located over a restricted area of the head surface. Proteins with similar molecular weight were labelled on spermatozoa from the cauda epididymidis, but not from the testis, by reaction with sodium boro[3H]hydride in the presence of galactose oxidase. However, failure to immunoprecipitate with antibodies to Proteins D and E and non-coincident migration on two-dimensional gel electrophoresis established the non-identity of these proteins. Compared with Proteins D and E, two other major epididymal secretory proteins (Proteins B and C of Mr 16 000) associated with spermatozoa to a relatively minor extent during epididymal transit.
大鼠附睾主细胞分泌的酸性糖蛋白的表征。
DOI：
10.1016/0005-2795(81)90170-7
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Lea,OA;French,FS
通讯作者：
French,FS
附睾运输过程中大鼠精子的表面变化。
影响因子：
3.6
作者：
Olson,GE;Danzo,BJ
通讯作者：
Danzo,BJ