A Subset of Polysaccharide Capsules in the Human Symbiont Bacteroides thetaiotaomicron Promote Increased Competitive Fitness in the Mouse Gut.
A Subset of Polysaccharide Capsules in the Human Symbiont Bacteroides thetaiotaomicron Promote Increased Competitive Fitness in the Mouse Gut.
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DOI：
10.1016/j.chom.2017.08.020
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发表时间：
2017-10-11
影响因子：
30.3
通讯作者：
Martens EC
中科院分区：
文献类型：
--
作者：
Porter NT;Canales P;Peterson DA;Martens EC
荚膜多糖（CPS）在保护细菌免受宿主和环境因素影响方面发挥着多种作用，许多共生细菌可以产生多种荚膜类型。为了更好地了解不同 CPS 在竞争性肠道定植中的作用，我们单独表达了人类肠道共生体 Bacteroides thetaiotaomicron 的八种不同胶囊。某些 CPS 在体内是最有利的，并且抗 CPS IgA 的增加与表达一种特定胶囊 CPS5 的菌株的适应性增加相关，表明它促进了适应性免疫的避免。能够在多个胶囊之间切换的菌株比表达除 CPS5 之外的任何单个胶囊的菌株更具竞争力。抗生素干扰后，只有野生型胶囊转换菌株留在肠道中，仅在具有完整适应性免疫的小鼠中转为显着表达 CPS5。这些数据表明，不同的胶囊使共生的肠道细菌能够在不同的生态位中茁壮成长，包括那些受免疫反应和抗生素干扰影响的细菌。共生拟杆菌编码多种荚膜多糖，但其作用仍不清楚。波特等人。鉴定出一种参与小鼠肠道竞争性定植的胶囊，有助于逃避适应性免疫反应。合成这种胶囊的基因经常在人类肠道样本中被发现。
Capsular polysaccharides (CPS) play multiple roles in protecting bacteria from host and environmental factors, and many commensal bacteria can produce multiple capsule types. To better understand the roles of different CPS in competitive intestinal colonization, we individually expressed the eight different capsules of the human gut symbiont Bacteroides thetaiotaomicron. Certain CPS were most advantageous in vivo, and increased anti-CPS IgA correlated with increased fitness of a strain expressing one particular capsule CPS5, suggesting that it promotes avoidance of adaptive immunity. A strain with the ability to switch between multiple capsules was more competitive than those expressing any single capsule except CPS5. After antibiotic perturbation, only the wild-type, capsule-switching strain remained in the gut, shifting to prominent expression of CPS5 only in mice with intact adaptive immunity. These data suggest that different capsules equip mutualistic gut bacteria with the ability to thrive in various niches, including those influenced by immune responses and antibiotic perturbations. Symbiotic Bacteroides species encode for multiple capsular polysaccharides, the roles of which have remained poorly defined. Porter et al. identify one capsule involved in competitive colonization of the mouse gut that assists in evading adaptive immune responses. The genes for synthesizing this capsule are frequently identified in human gut samples.
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影响因子：
30.3
作者：
Martens EC;Chiang HC;Gordon JI
通讯作者：
Gordon JI
DOI：
10.1074/jbc.m109.008094
发表时间：
2009-07-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Martens EC;Roth R;Heuser JE;Gordon JI
通讯作者：
Gordon JI
影响因子：
6.4
作者：
Cameron EA;Kwiatkowski KJ;Lee BH;Hamaker BR;Koropatkin NM;Martens EC
通讯作者：
Martens EC
影响因子：
56.9
作者：
Coyne, MJ;Reinap, B;Comstock, LE
通讯作者：
Comstock, LE
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI