Species‐dependent antigenicity of the 34‐kDa glycoprotein found on the membrane of various primate lymphocytes transformed by human t‐cell leukemia virus type‐I (HTLV‐I) and simian T‐cell leukemia virus (STLV‐I)
Species‐dependent antigenicity of the 34‐kDa glycoprotein found on the membrane of various primate lymphocytes transformed by human t‐cell leukemia virus type‐I (HTLV‐I) and simian T‐cell leukemia virus (STLV‐I)
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在人 T 细胞白血病病毒 I 型 (HTLV-I) 和猿猴 T 细胞白血病病毒 (STLV-I) 转化的各种灵长类淋巴细胞膜上发现的 34-kDa 糖蛋白的物种依赖性抗原性
DOI：
10.1002/ijc.2910410213
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发表时间：
1988
影响因子：
6.4
通讯作者：
Y. Hinuma
中科院分区：
文献类型：
--
作者：
H. Tozawa;S. Andoh;Yoshinaga Takayama;Yuetsu Tanaka;Bunsho Lee;Hideo Nakamura;M. Hayami;Y. Hinuma
制备了16种针对人T细胞白血病病毒（HTLV - I）感染细胞表面TA34抗原的单克隆抗体（mab）。这些单克隆抗体和先前制备的抗TA34单克隆抗体（TAG34）不仅在HTLV‐1感染的细胞中识别34‐kDa肽，而且在感染类人猿T细胞白血病病毒（STLV‐1）的细胞中也识别34‐kDa肽，后者在抗原性和基因结构上与HTLV‐1相似。单抗放射免疫沉淀（RIP）试验显示TA34抗原至少有3个重叠的表位组。采用9种抗TA34单克隆抗体进行免疫荧光染色，研究了不同灵长类动物HTLV - I感染细胞的TA34抗原的抗原性。来自人类、猿猴和旧大陆猴的细胞与所有这些抗体都有反应，而来自新大陆猴的细胞被大多数抗体染色，但几乎没有被剩余的2 （5A8和TAG34）染色。在感染STLV‐I的各种灵长类细胞中也得到了类似的结果。所有17种单克隆抗体在tunicamycin存在下培养的HTLV - I感染细胞中识别22 - kDa肽。当与1% 2巯基乙醇在37°C下ph 7.2孵育时，TA34抗原失去了与TAG34的反应性，这表明抗原具有分子内S - S键。在RIP试验中，20例成人T细胞白血病（ATL）患者血清与TA34抗原无反应。
Sixteen monoclonal antibodies (MAbs) against TA34 antigen found on the surface of human T‐cell leukemia virus type‐I (HTLV‐I) infected cells were prepared. These MAbs and one previously prepared anti‐TA34 MAb (TAG34) recognized 34‐kDa peptide not only in HTLV‐I‐infected cells, but also in cells infected with simian T‐cell leukemia virus (STLV‐I), which is analogous in antigenicity and gene structure to HTLV‐I. Radioimmuno‐precipitation (RIP) tests with the MAbs showed that TA34 antigen had at least 3 overlapping epitope groups. The antigenicities of the TA34 antigens of HTLV‐I‐infected cells derived from various primates were investigated by immunofluorescence staining using 9 anti‐TA34 MAbs. Cells from humans, apes and Old World monkeys reacted with all these antibodies, whereas cells from New World monkeys were stained by most of the antibodies, but little if at all by the remaining 2 (5A8 and TAG34). Similar results were obtained with various primate cells infected with STLV‐I. All 17 MAbs used recognized a 22‐kDa peptide in HTLV‐I‐infected cells cultured in the presence of tunicamycin. When incubated with 1% 2‐mercaptoethanol at ph 7.2 at 37°C, TA34 antigen lost its reactivity with TAG34, suggesting that the antigen has an intramolecular S‐S bond. Twenty sera of adult T‐cell leukemia (ATL) patients did not react with TA34 antigen in RIP tests.
DOI：
10.1073/pnas.79.6.2031
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
YOSHIDA, M;MIYOSHI, I;HINUMA, Y
通讯作者：
HINUMA, Y