Proteomic analysis of diabetic retinas.
Proteomic analysis of diabetic retinas.
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糖尿病视网膜的蛋白质组学分析。
DOI：
10.3389/fendo.2023.1229089
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
作为一种代谢性疾病，糖尿病经常导致健康并发症，如心力衰竭、肾病、神经疾病和视力丧失。糖尿病视网膜病变(DR)影响全球多达1亿人。DR的机制很复杂，已知会影响视网膜的神经和血管成分。虽然该领域的最新进展已经确定了参与DR发病机制的主要细胞信号，但关于蛋白质翻译后修饰(PTM)-已知的定义蛋白质的定位、功能和活性-在整个糖尿病视网膜中的报道很少。蛋白质糖基化是碳水化合物在蛋白质上的加成反应，它可以影响蛋白质的许多属性，包括折叠、稳定性、功能和亚细胞定位。O-连接糖基化是将糖加到氨基酸的氧原子上，在其侧链上有一个自由氧原子(即苏氨酸、丝氨酸)。到目前为止，已经描述了100多种先天性糖基化障碍。然而，还没有研究确定视网膜O-连接糖蛋白组在健康或疾病中的作用。由于迫切需要加快在糖尿病视网膜中发现PTM组学，我们确定了对照组和糖尿病小鼠的蛋白质水平和视网膜O-糖蛋白组学的全球变化。我们使用基于液相色谱/质谱仪的蛋白质组学和高通量筛选来鉴定野生型和糖尿病小鼠视网膜中差异表达的蛋白质和差异O-糖基化的蛋白质。野生型和糖尿病小鼠视网膜中蛋白质和差异糖基化蛋白质的全球表达水平的变化已经被发现。我们提供的证据表明，糖尿病改变了视网膜中代谢和突触蛋白的整体表达水平和O-糖基化。在这里，我们报告了糖尿病小鼠视网膜蛋白质组的变化。我们强调参与新陈代谢过程、维持细胞结构、运输和神经元过程的全球蛋白质的变化。然后，我们显示了糖尿病视网膜中个别蛋白质的O-连接糖基化的变化。
As a metabolic disease, diabetes often leads to health complications such as heart failure, nephropathy, neurological disorders, and vision loss. Diabetic retinopathy (DR) affects as many as 100 million people worldwide. The mechanism of DR is complex and known to impact both neural and vascular components in the retina. While recent advances in the field have identified major cellular signaling contributing to DR pathogenesis, little has been reported on the protein post-translational modifications (PTM) - known to define protein localization, function, and activity - in the diabetic retina overall. Protein glycosylation is the enzymatic addition of carbohydrates to proteins, which can influence many protein attributes including folding, stability, function, and subcellular localization. O-linked glycosylation is the addition of sugars to an oxygen atom in amino acids with a free oxygen atom in their side chain (i.e., threonine, serine). To date, more than 100 congenital disorders of glycosylation have been described. However, no studies have identified the retinal O-linked glycoproteome in health or disease. With a critical need to expedite the discovery of PTMomics in diabetic retinas, we identified both global changes in protein levels and the retinal O-glycoproteome of control and diabetic mice. We used liquid chromatography/mass spectrometry-based proteomics and high throughput screening to identify proteins differentially expressed and proteins differentially O-glycosylated in the retinas of wildtype and diabetic mice. Changes in both global expression levels of proteins and proteins differentially glycosylated in the retinas of wild-type and diabetic mice have been identified. We provide evidence that diabetes shifts both global expression levels and O-glycosylation of metabolic and synaptic proteins in the retina. Here we report changes in the retinal proteome of diabetic mice. We highlight alterations in global proteins involved in metabolic processes, maintaining cellular structure, trafficking, and neuronal processes. We then showed changes in O-linked glycosylation of individual proteins in the diabetic retina.
影响因子：
5.3
作者：
Starr, Christopher R.;Gorbatyuk, Marina S.
通讯作者：
Gorbatyuk, Marina S.
异常糖基化增强可溶性钙网蛋白的免疫刺激活性
DOI：
10.3390/molecules23030523
发表时间：
2018-02-27
期刊：
Molecules (Basel, Switzerland)
影响因子：
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作者：
Gong FY;Gong Z;Duo CC;Wang J;Hong C;Gao XM
通讯作者：
Gao XM
影响因子：
5.2
作者：
通讯作者：
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