Automated screening procedure for high-throughput generation of antibody fragments.
Automated screening procedure for high-throughput generation of antibody fragments.
复制标题
高通量生成抗体片段的自动筛选程序。
作者：
J. Hallborn;R. Carlsson
在新兴的蛋白质组学领域，迫切需要捕获分子，如抗体，以微阵列形式检测蛋白质组或部分蛋白质组。通过组合文库，如单链抗体片段（scFv）或Fab片段的噬菌体展示文库，可以获得提供多种结合物的合适来源。为了从n-CoDeR库中以高通量找到新的粘合剂，我们使用机器人技术自动化了筛选过程。自动化系统配置为每天筛选数以万计的克隆，以靶抗原的各种格式，包括肽和可溶性蛋白，以及细胞结合的目标；因此，它的设计很好地满足了蛋白质组学领域的需求。
In the emerging field of proteomics, there is an urgent need for catcher molecules such as antibodies for detecting the proteome or parts of the proteome in a microarray format. A suitable source for providing a large diversity of binders is obtained by combinatorial libraries, such as phage display libraries of single chain antibody fragments (scFv) or Fab fragments. To find novel binders from the n-CoDeR libraries with a high throughput, we have automated the screening process with robotics. The automated system is configured to screen tens of thousands of clones per day to target antigens in various formats, including peptides and soluble proteins, as well as cell-bound targets; thus, it is well designed to meet demands from the proteomics area.
影响因子：
56.9
作者：
SMITH, GP
通讯作者：
SMITH, GP