Fate of amnion-derived stem cells transplanted to the fetal rat brain: migration, survival and differentiation.
Fate of amnion-derived stem cells transplanted to the fetal rat brain: migration, survival and differentiation.
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DOI：
10.1111/j.1582-4934.2008.00180.x
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发表时间：
2008-08
影响因子：
5.3
通讯作者：
Woodbury D
中科院分区：
文献类型：
--
作者：
Marcus AJ;Coyne TM;Black IB;Woodbury D
我们最近的特点是从啮齿动物羊膜分离的干细胞群称为羊膜源性干细胞（ADSCs）。体外ADSC分化成代表所有三个胚胎层的细胞类型，包括神经细胞。在这项研究中，我们评估了脂肪干细胞在子宫内移植到发育中的大鼠脑中后的体内神经外胚层潜能。将表达绿色荧光蛋白的ADSC克隆系注入发育中的胚胎15.5天大鼠脑的端脑室中。在E17.5，供体细胞主要以球体存在于心室中，亚群融合到心室壁，表明进入脑实质的模式。通过E21.5绿色荧光蛋白（GFP）ADSCs迁移到一些脑区。在出生后的时间点检查显示，供体ADSCs表达波形蛋白和巢蛋白。亚组的移植脂肪干细胞达到神经元形态，虽然没有神经或胶质细胞分化的免疫组化证据。一些供体细胞在血管周围迁移并分化为假定的内皮细胞。子宫内移植的供体ADSC在受体中存在至成年，没有免疫排斥或肿瘤形成的证据。长期存活可能表明在治疗不需要分化为神经细胞类型以获得临床益处的疾病中的效用。
We have recently characterized a stem cell population isolated from the rodent amniotic membrane termed amnion-derived stem cells (ADSCs). In vitro ADSCs differentiate into cell types representing all three embryonic layers, including neural cells. In this study we evaluated the neuroectodermal potential of ADSCs in vivo after in utero transplantation into the developing rat brain. A clonal line of green fluorescent protein-expressing ADSCs were infused into the telencephalic ventricles of the developing embryonic day 15.5 rat brain. At E17.5 donor cells existed primarily as spheres in the ventricles with subsets fused to the ventricular walls, suggesting a mode of entry into the brain parenchyma. By E21.5 green fluorescent protein (GFP) ADSCs migrated to a number of brain regions. Examination at postnatal time points revealed that donor ADSCs expressed vimentin and nestin. Subsets of transplanted ADSCs attained neuronal morphologies, although there was no immunohistochemical evidence of neural or glial differentiation. Some donor cells migrated around blood vessels and differentiated into putative endothelial cells. Donor ADSCs transplanted in utero were present in recipients into adulthood with no evidence of immunological rejection or tumour formation. Long-term survival may suggest utility in the treatment of disorders where differentiation to a neural cell type is not required for clinical benefit.
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影响因子：
2.9
作者：
Marcus, Akiva J.;Coyne, Thomas M.;Black, Ira B.
通讯作者：
Black, Ira B.
DOI：
10.1073/pnas.0608249103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Lee, Ryang Hwa;Seo, Min Jeong;Prockop, Darwin J.
通讯作者：
Prockop, Darwin J.
影响因子：
6.5
作者：
Erices, A;Conget, P;Minguell, JJ
通讯作者：
Minguell, JJ
影响因子：
20.3
作者：
Casal, ML;Wolfe, JH
通讯作者：
Wolfe, JH
影响因子：
5.3
作者：
Yasuhara, Takao;Matsukawa, Noriyuki;Borlongan, Cesario V.
通讯作者：
Borlongan, Cesario V.